Single molecule high-throughput footprinting of small and large DNA ligands

Single molecule high-throughput footprinting of small and large DNA ligands

Mapping the sequence specificity of DNA ligands remains a challenge, particularly for small drugs. Here the authors develop a parallelized single molecule magnetic tweezers approach using engineered DNA hairpins that can detect sequence selectivity, thermodynamics and kinetics of binding for small d...

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Bibliographic Details
Main Authors: Maria Manosas, Joan Camunas-Soler, Vincent Croquette, Felix Ritort
Format: Article
Language:English
Published: Nature Publishing Group 2017-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-017-00379-w
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spelling doaj-c9ac960182d347a292e4a7983f566da92021-01-31T12:26:17ZengNature Publishing GroupNature Communications2041-17232017-08-018111210.1038/s41467-017-00379-wSingle molecule high-throughput footprinting of small and large DNA ligandsMaria Manosas0Joan Camunas-Soler1Vincent Croquette2Felix Ritort3Small Biosystems Lab, Departament de Fsica de la Matèria Condensada, Facultat de Física, Universitat de BarcelonaSmall Biosystems Lab, Departament de Fsica de la Matèria Condensada, Facultat de Física, Universitat de BarcelonaENS, PSL Research University, UPMC, Université Paris Diderot, Dept. de Physique et IBENS, CNRS UMR-8550, LPSSmall Biosystems Lab, Departament de Fsica de la Matèria Condensada, Facultat de Física, Universitat de BarcelonaMapping the sequence specificity of DNA ligands remains a challenge, particularly for small drugs. Here the authors develop a parallelized single molecule magnetic tweezers approach using engineered DNA hairpins that can detect sequence selectivity, thermodynamics and kinetics of binding for small drugs and large proteins.https://doi.org/10.1038/s41467-017-00379-w
collection DOAJ
language English
format Article
sources DOAJ
author Maria Manosas
Joan Camunas-Soler
Vincent Croquette
Felix Ritort
spellingShingle Maria Manosas
Joan Camunas-Soler
Vincent Croquette
Felix Ritort
Single molecule high-throughput footprinting of small and large DNA ligands
Nature Communications
author_facet Maria Manosas
Joan Camunas-Soler
Vincent Croquette
Felix Ritort
author_sort Maria Manosas
title Single molecule high-throughput footprinting of small and large DNA ligands
title_short Single molecule high-throughput footprinting of small and large DNA ligands
title_full Single molecule high-throughput footprinting of small and large DNA ligands
title_fullStr Single molecule high-throughput footprinting of small and large DNA ligands
title_full_unstemmed Single molecule high-throughput footprinting of small and large DNA ligands
title_sort single molecule high-throughput footprinting of small and large dna ligands
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2017-08-01
description Mapping the sequence specificity of DNA ligands remains a challenge, particularly for small drugs. Here the authors develop a parallelized single molecule magnetic tweezers approach using engineered DNA hairpins that can detect sequence selectivity, thermodynamics and kinetics of binding for small drugs and large proteins.
url https://doi.org/10.1038/s41467-017-00379-w
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AT joancamunassoler singlemoleculehighthroughputfootprintingofsmallandlargednaligands
AT vincentcroquette singlemoleculehighthroughputfootprintingofsmallandlargednaligands
AT felixritort singlemoleculehighthroughputfootprintingofsmallandlargednaligands
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