Single molecule high-throughput footprinting of small and large DNA ligands

Single molecule high-throughput footprinting of small and large DNA ligands

Mapping the sequence specificity of DNA ligands remains a challenge, particularly for small drugs. Here the authors develop a parallelized single molecule magnetic tweezers approach using engineered DNA hairpins that can detect sequence selectivity, thermodynamics and kinetics of binding for small d...

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Bibliographic Details
Main Authors: Maria Manosas, Joan Camunas-Soler, Vincent Croquette, Felix Ritort
Format: Article
Language:English
Published: Nature Publishing Group 2017-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-017-00379-w
Description
Summary:Mapping the sequence specificity of DNA ligands remains a challenge, particularly for small drugs. Here the authors develop a parallelized single molecule magnetic tweezers approach using engineered DNA hairpins that can detect sequence selectivity, thermodynamics and kinetics of binding for small drugs and large proteins.
ISSN:2041-1723

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