Increased serum concentrations of IL-1 beta, IL-21 and Th17 cells in overweight patients with rheumatoid arthritis

• Main Authors: Hirofumi Shoda, Yasuo Nagafuchi, Yumi Tsuchida, Keiichi Sakurai, Shuji Sumitomo, Keishi Fujio, Kazuhiko Yamamoto
• Format: Article
• Language: English
• Published: BMC 2017-05-01
• Series: Arthritis Research & Therapy
• Subjects: Rheumatoid arthritis; Body mass index; Th17; IL-1β; IL-21
• Online Access: http://link.springer.com/article/10.1186/s13075-017-1308-y

Abstract Backgrounds Obesity is associated with worse disease activity and drug responses in patients with rheumatoid arthritis (RA). However, the immunological mechanisms responsible for the relationship between RA and obesity have not yet been clarified in detail. This study aimed to elucidate the immunological mechanisms contributing to the pathogenesis of RA in overweight patients. Methods The frequencies of CD4+ T cell, B cell and monocyte subsets were analyzed in RA (n = 81) and healthy donors (n = 99) by flow cytometry, and were compared between three groups (body mass index (BMI) <20,  ≥20 to 25, >25). Serum cytokines were measured using multiplex ELISA. Gene expression was analyzed by quantitative PCR. Clinical information was extracted from medical records. Results The frequencies of T helper (Th)17 (CD4+CD45RA-CXCR5-CXCR3-CCR6+) cells and plasmablasts (PB) were significantly increased in patients with RA with BMI >25. Significant correlation was observed between BMI and Th17 cells in patients with RA. No significant differences in cell frequencies between the three BMI groups were observed in the healthy donors. Serum interleukin (IL)-1β and IL-21 significantly correlated with BMI in RA patients. Gene expression patterns in Th17 cells from overweight patients with RA showed the characteristics of pathogenic Th17 cells. Conclusions Quantitative and qualitative changes in Th17 cells were characteristic in overweight patients with RA.