A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegans

A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegans

The residue rich in protein (up to 88.78%) obtained from round scad (Decapterus maruadsi) after oil extraction, was hydrolyzed by Alcalase to produce antioxidative peptides. The hydrolysate was separated by ultrafiltration, Sephadex G-15 and RP-HPLC, and the active fractions were screened by 2,2-dip...

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Main Authors: Haiqiang Chen, Shuang Wang, Aimei Zhou, Jianyin Miao, Jingyi Liu, Soottawat Benjakul
Format: Article
Language:English
Published: Elsevier 2020-05-01
Series:Journal of Functional Foods
Subjects:
Antioxidative peptide
Purification
Caenorhabditis elegans
Anti-aging
Round scad
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464620301316
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spelling doaj-c94edcd097954274b0661d1363ce193b2021-04-30T07:18:41ZengElsevierJournal of Functional Foods1756-46462020-05-0168103907A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegansHaiqiang Chen0Shuang Wang1Aimei Zhou2Jianyin Miao3Jingyi Liu4Soottawat Benjakul5College of Food Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, Guangzhou 510642, China; Corresponding author at: College of Food Science, South China Agricultural University, Guangzhou 510642, China.College of Food Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, Guangzhou 510642, ChinaDepartment of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandThe residue rich in protein (up to 88.78%) obtained from round scad (Decapterus maruadsi) after oil extraction, was hydrolyzed by Alcalase to produce antioxidative peptides. The hydrolysate was separated by ultrafiltration, Sephadex G-15 and RP-HPLC, and the active fractions were screened by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline- 6 sulphonic acid) diammonium salt (ABTS), reducing power and oxygen radical absorbance capacity (ORAC). The obtained peptide with the highest antioxidative activity was then analyzed by HPLC and LC-MS/MS and its anti-aging effect was examined using Caenorhabditis elegans (C. elegans) model. The peptide (Ile-Leu-Gly-Ala-Thr-Ile-Asp-Asn-Ser-Lys, 1153.4 Da), identified by LC-MS/MS showed the highest antioxidant activity. C. elegans fed with the selected peptide had longer lifespan, higher survival rate, superoxide dismutase (SOD) and catalase (CAT) activities than the control group (P＜0.05), demonstrating the promising anti-aging effects of the antioxidant peptide from defatted round scad.http://www.sciencedirect.com/science/article/pii/S1756464620301316Antioxidative peptidePurificationCaenorhabditis elegansAnti-agingRound scad
collection DOAJ
language English
format Article
sources DOAJ
author Haiqiang Chen
Shuang Wang
Aimei Zhou
Jianyin Miao
Jingyi Liu
Soottawat Benjakul
spellingShingle Haiqiang Chen
Shuang Wang
Aimei Zhou
Jianyin Miao
Jingyi Liu
Soottawat Benjakul
A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegans
Journal of Functional Foods
Antioxidative peptide
Purification
Caenorhabditis elegans
Anti-aging
Round scad
author_facet Haiqiang Chen
Shuang Wang
Aimei Zhou
Jianyin Miao
Jingyi Liu
Soottawat Benjakul
author_sort Haiqiang Chen
title A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegans
title_short A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegans
title_full A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegans
title_fullStr A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegans
title_full_unstemmed A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegans
title_sort novel antioxidant peptide purified from defatted round scad (decapterus maruadsi) protein hydrolysate extends lifespan in caenorhabditis elegans
publisher Elsevier
series Journal of Functional Foods
issn 1756-4646
publishDate 2020-05-01
description The residue rich in protein (up to 88.78%) obtained from round scad (Decapterus maruadsi) after oil extraction, was hydrolyzed by Alcalase to produce antioxidative peptides. The hydrolysate was separated by ultrafiltration, Sephadex G-15 and RP-HPLC, and the active fractions were screened by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline- 6 sulphonic acid) diammonium salt (ABTS), reducing power and oxygen radical absorbance capacity (ORAC). The obtained peptide with the highest antioxidative activity was then analyzed by HPLC and LC-MS/MS and its anti-aging effect was examined using Caenorhabditis elegans (C. elegans) model. The peptide (Ile-Leu-Gly-Ala-Thr-Ile-Asp-Asn-Ser-Lys, 1153.4 Da), identified by LC-MS/MS showed the highest antioxidant activity. C. elegans fed with the selected peptide had longer lifespan, higher survival rate, superoxide dismutase (SOD) and catalase (CAT) activities than the control group (P＜0.05), demonstrating the promising anti-aging effects of the antioxidant peptide from defatted round scad.
topic Antioxidative peptide
Purification
Caenorhabditis elegans
Anti-aging
Round scad
url http://www.sciencedirect.com/science/article/pii/S1756464620301316
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