Comprehensive Analysis of the Chitinase Gene Family in Cucumber (<i>Cucumis sativus</i> L.): From Gene Identification and Evolution to Expression in Response to <i>Fusarium oxysporum</i>

Comprehensive Analysis of the Chitinase Gene Family in Cucumber (<i>Cucumis sativus</i> L.): From Gene Identification and Evolution to Expression in Response to <i>Fusarium oxysporum</i>

Chitinases, a subgroup of pathogenesis-related proteins, are responsible for catalyzing the hydrolysis of chitin. Accumulating reports indicate that chitinases play a key role in plant defense against chitin-containing pathogens and are therefore good targets for defense response studies. Here, we u...

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Main Authors: Ezra S. Bartholomew, Kezia Black, Zhongxuan Feng, Wan Liu, Nan Shan, Xiao Zhang, Licai Wu, Latoya Bailey, Ning Zhu, Changhong Qi, Huazhong Ren, Xingwang Liu
Format: Article
Language:English
Published: MDPI AG 2019-10-01
Series:International Journal of Molecular Sciences
Subjects:
<i>cucumis sativus</i>
chitinase
<i>fusarium oxysporum</i>
gene family
gene expression
Online Access:https://www.mdpi.com/1422-0067/20/21/5309
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spelling doaj-c9238e22c1cf4600b9778c09d4b815f42020-11-25T02:33:14ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-10-012021530910.3390/ijms20215309ijms20215309Comprehensive Analysis of the Chitinase Gene Family in Cucumber (<i>Cucumis sativus</i> L.): From Gene Identification and Evolution to Expression in Response to <i>Fusarium oxysporum</i>Ezra S. Bartholomew0Kezia Black1Zhongxuan Feng2Wan Liu3Nan Shan4Xiao Zhang5Licai Wu6Latoya Bailey7Ning Zhu8Changhong Qi9Huazhong Ren10Xingwang Liu11Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, ChinaChangping Agricultural Technology Service Center, Beijing 102200, ChinaChangping Agricultural Technology Service Center, Beijing 102200, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing 100193, ChinaChitinases, a subgroup of pathogenesis-related proteins, are responsible for catalyzing the hydrolysis of chitin. Accumulating reports indicate that chitinases play a key role in plant defense against chitin-containing pathogens and are therefore good targets for defense response studies. Here, we undertook an integrated bioinformatic and expression analysis of the cucumber chitinases gene family to identify its role in defense against <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i>. A total of 28 putative chitinase genes were identified in the cucumber genome and classified into five classes based on their conserved catalytic and binding domains. The expansion of the chitinase gene family was due mainly to tandem duplication events. The expression pattern of chitinase genes was organ-specific and 14 genes were differentially expressed in response to <i>F. oxysporum</i> challenge of fusarium wilt-susceptible and resistant lines. Furthermore, a class I chitinase, <i>CsChi23</i>, was constitutively expressed at high levels in the resistant line and may play a crucial role in building a basal defense and activating a rapid immune response against <i>F. oxysporum</i>. Whole-genome re-sequencing of both lines provided clues for the diverse expression patterns observed. Collectively, these results provide useful genetic resource and offer insights into the role of chitinases in cucumber-<i>F. oxysporum</i> interaction.https://www.mdpi.com/1422-0067/20/21/5309<i>cucumis sativus</i>chitinase<i>fusarium oxysporum</i>gene familygene expression
collection DOAJ
language English
format Article
sources DOAJ
author Ezra S. Bartholomew
Kezia Black
Zhongxuan Feng
Wan Liu
Nan Shan
Xiao Zhang
Licai Wu
Latoya Bailey
Ning Zhu
Changhong Qi
Huazhong Ren
Xingwang Liu
spellingShingle Ezra S. Bartholomew
Kezia Black
Zhongxuan Feng
Wan Liu
Nan Shan
Xiao Zhang
Licai Wu
Latoya Bailey
Ning Zhu
Changhong Qi
Huazhong Ren
Xingwang Liu
Comprehensive Analysis of the Chitinase Gene Family in Cucumber (<i>Cucumis sativus</i> L.): From Gene Identification and Evolution to Expression in Response to <i>Fusarium oxysporum</i>
International Journal of Molecular Sciences
<i>cucumis sativus</i>
chitinase
<i>fusarium oxysporum</i>
gene family
gene expression
author_facet Ezra S. Bartholomew
Kezia Black
Zhongxuan Feng
Wan Liu
Nan Shan
Xiao Zhang
Licai Wu
Latoya Bailey
Ning Zhu
Changhong Qi
Huazhong Ren
Xingwang Liu
author_sort Ezra S. Bartholomew
title Comprehensive Analysis of the Chitinase Gene Family in Cucumber (<i>Cucumis sativus</i> L.): From Gene Identification and Evolution to Expression in Response to <i>Fusarium oxysporum</i>
title_short Comprehensive Analysis of the Chitinase Gene Family in Cucumber (<i>Cucumis sativus</i> L.): From Gene Identification and Evolution to Expression in Response to <i>Fusarium oxysporum</i>
title_full Comprehensive Analysis of the Chitinase Gene Family in Cucumber (<i>Cucumis sativus</i> L.): From Gene Identification and Evolution to Expression in Response to <i>Fusarium oxysporum</i>
title_fullStr Comprehensive Analysis of the Chitinase Gene Family in Cucumber (<i>Cucumis sativus</i> L.): From Gene Identification and Evolution to Expression in Response to <i>Fusarium oxysporum</i>
title_full_unstemmed Comprehensive Analysis of the Chitinase Gene Family in Cucumber (<i>Cucumis sativus</i> L.): From Gene Identification and Evolution to Expression in Response to <i>Fusarium oxysporum</i>
title_sort comprehensive analysis of the chitinase gene family in cucumber (<i>cucumis sativus</i> l.): from gene identification and evolution to expression in response to <i>fusarium oxysporum</i>
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2019-10-01
description Chitinases, a subgroup of pathogenesis-related proteins, are responsible for catalyzing the hydrolysis of chitin. Accumulating reports indicate that chitinases play a key role in plant defense against chitin-containing pathogens and are therefore good targets for defense response studies. Here, we undertook an integrated bioinformatic and expression analysis of the cucumber chitinases gene family to identify its role in defense against <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i>. A total of 28 putative chitinase genes were identified in the cucumber genome and classified into five classes based on their conserved catalytic and binding domains. The expansion of the chitinase gene family was due mainly to tandem duplication events. The expression pattern of chitinase genes was organ-specific and 14 genes were differentially expressed in response to <i>F. oxysporum</i> challenge of fusarium wilt-susceptible and resistant lines. Furthermore, a class I chitinase, <i>CsChi23</i>, was constitutively expressed at high levels in the resistant line and may play a crucial role in building a basal defense and activating a rapid immune response against <i>F. oxysporum</i>. Whole-genome re-sequencing of both lines provided clues for the diverse expression patterns observed. Collectively, these results provide useful genetic resource and offer insights into the role of chitinases in cucumber-<i>F. oxysporum</i> interaction.
topic <i>cucumis sativus</i>
chitinase
<i>fusarium oxysporum</i>
gene family
gene expression
url https://www.mdpi.com/1422-0067/20/21/5309
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