Glutamic acid and folic acid production in aerobic and anaerobic probiotics

• Main Authors: Zohre Taghi Abadi, Mehrana Koohi Dehkordi, Giti Emtiazi
• Format: Article
• Language: English
• Published: University of Isfahan 2018-03-01
• Series: Biological Journal of Microorganism
• Subjects: Bifidobacteria; Bifidobacterium bifidum; Sporolactobacillus; Amino acid; High Pressure Liquid Chromatography; Thin Layer Chromatography
• Online Access: http://bjm.ui.ac.ir/article_21748_beec67025fadbc3c660f9f0d154312d4.pdf

Introduction:From an industrial application or commercial point of view, glutamic acid is one of the most important amino acids and its microbial production has been reported from some bacteria. Regarding the role of probiotics to modulate human health and the ever-increasing demand of prebiotics in the food industry, in the current study, production of glutamic acid and folic acid from three probiotic bacteria (Bifidobacterium, Bifidobacterium bifidum, Sporolactobacillus) was evaluated for the first time. Materials and methods: MRS broth and exclusive media was used for probiotic culture. The glutamic acid was identified using thin-layer chromatography and folic acid production was measured by folate kit. Each bacterium in terms of quality and quantity were measured by high pressure liquid chromatography. Results: Production of glutamic acid confirmed is based on the thin layer chromatography analysis and high pressure liquid chromatography results. In addition, it was observed that all three probiotics produce folic acid. The prevalence of folate in Bifidobacterium was measured as 315 mg/ml that was more than two other bacteria. Discussion and conclusion: To the best of our knowledge, this is the first report of microbial production of glutamic acid and folate from the probiotic bacteria. These beneficial bacteria can be used as a good source for mass production of these valuable compounds.