Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature

Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature

Summary: Current protocols for storage of white blood cells (WBCs) rely on constant refrigeration. The protocol described below explains the preparation of a fixative combination saline (FCS) formulation, which allows fixation of human WBCs and lysis of red blood cells and platelets (at ambient temp...

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Bibliographic Details
Main Authors: Rajesh K. Chaurasia, Kapil B. Shirsath, Balvinder K. Sapra
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Health Sciences
Clinical Protocol
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005402
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spelling doaj-c90ca5364fa54aaa8a614852522ff32c2021-09-17T04:37:58ZengElsevierSTAR Protocols2666-16672021-12-0124100834Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperatureRajesh K. Chaurasia0Kapil B. Shirsath1Balvinder K. Sapra2Radiological Physics and Advisory Division, Bhabha Atomic Research Centre (BARC), Mumbai, Maharashtra 400094, India; Homi Bhabha National Institute (HBNI), Mumbai, Maharashtra 400094, IndiaRadiological Physics and Advisory Division, Bhabha Atomic Research Centre (BARC), Mumbai, Maharashtra 400094, IndiaRadiological Physics and Advisory Division, Bhabha Atomic Research Centre (BARC), Mumbai, Maharashtra 400094, India; Homi Bhabha National Institute (HBNI), Mumbai, Maharashtra 400094, India; Corresponding authorSummary: Current protocols for storage of white blood cells (WBCs) rely on constant refrigeration. The protocol described below explains the preparation of a fixative combination saline (FCS) formulation, which allows fixation of human WBCs and lysis of red blood cells and platelets (at ambient temperature, 4–35oC) in whole blood samples in one step. FCS can be used for storing and transporting blood at ambient temperatures for up to 4 months, without altering the nuclear morphology and genomic integrity of WBCs.http://www.sciencedirect.com/science/article/pii/S2666166721005402Health SciencesClinical Protocol
collection DOAJ
language English
format Article
sources DOAJ
author Rajesh K. Chaurasia
Kapil B. Shirsath
Balvinder K. Sapra
spellingShingle Rajesh K. Chaurasia
Kapil B. Shirsath
Balvinder K. Sapra
Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
STAR Protocols
Health Sciences
Clinical Protocol
author_facet Rajesh K. Chaurasia
Kapil B. Shirsath
Balvinder K. Sapra
author_sort Rajesh K. Chaurasia
title Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
title_short Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
title_full Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
title_fullStr Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
title_full_unstemmed Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
title_sort protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-12-01
description Summary: Current protocols for storage of white blood cells (WBCs) rely on constant refrigeration. The protocol described below explains the preparation of a fixative combination saline (FCS) formulation, which allows fixation of human WBCs and lysis of red blood cells and platelets (at ambient temperature, 4–35oC) in whole blood samples in one step. FCS can be used for storing and transporting blood at ambient temperatures for up to 4 months, without altering the nuclear morphology and genomic integrity of WBCs.
topic Health Sciences
Clinical Protocol
url http://www.sciencedirect.com/science/article/pii/S2666166721005402
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AT kapilbshirsath protocolforonestepselectivelysisofredbloodcellsandplateletswithlongtermpreservationofwhitebloodcellshumanatambienttemperature
AT balvinderksapra protocolforonestepselectivelysisofredbloodcellsandplateletswithlongtermpreservationofwhitebloodcellshumanatambienttemperature
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