Serological Number for Characterization of Circulating Antibodies

• Main Authors: Andrea Palermo, Alexander Nesterov-Mueller
• Format: Article
• Language: English
• Published: MDPI AG 2019-01-01
• Series: International Journal of Molecular Sciences
• Subjects: antibodies; binding motif; humoral memory; vaccination
• Online Access: https://www.mdpi.com/1422-0067/20/3/604

The dissociation constant of the circulating IgG antibodies is suggested to be proportional to the partial concentrations of these antibodies in blood serum in equilibrium. This coefficient, called serological number, is a dimensionless parameter and may be equal for all antibodies in a serum. Based on the serological number, we derived the equilibrium equation of the humoral immune system which allows estimating the number of different binding motifs in a serum. This equation also allows estimating the number of binding motifs of posttranslational and conformational nature. The feasibility of measuring the serological number via peptide arrays was demonstrated. Fifteen peptides with unique binding motifs were incubated and stained with the blood serum of a healthy adult at different dilutions. From these experiments, the serological number was determined. The serological number may explain the pre-existing antibody response after vaccination.