Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions

Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions

Summary: Labile heme is present in the cells at very low concentrations, either unbound or loosely bound to molecules, and accessible for signaling as alarmin. Our recent work suggests that extracellular heme can be taken up and detected in the nuclei of cancer cells. Here, we describe the detailed...

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Bibliographic Details
Main Authors: Giacomo Canesin, Lubica Janovicova, Barbara Wegiel
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell-based Assays
Cell separation/fractionation
Cancer
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001982
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spelling doaj-c8aa03b2f940427a85dac339e21c0f712021-06-21T04:25:23ZengElsevierSTAR Protocols2666-16672021-06-0122100491Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractionsGiacomo Canesin0Lubica Janovicova1Barbara Wegiel2Department of Surgery, Division of Surgical Oncology, Cancer Research Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02214, USADepartment of Surgery, Division of Surgical Oncology, Cancer Research Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02214, USADepartment of Surgery, Division of Surgical Oncology, Cancer Research Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02214, USA; Corresponding authorSummary: Labile heme is present in the cells at very low concentrations, either unbound or loosely bound to molecules, and accessible for signaling as alarmin. Our recent work suggests that extracellular heme can be taken up and detected in the nuclei of cancer cells. Here, we describe the detailed protocol for detection of labile and total heme in prostate cancer cells and its measurement in subcellular compartments in vitro. The protocol can be adapted to be used for other cell types.For complete details on the use and execution of this protocol, please refer to Canesin et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721001982Cell BiologyCell-based AssaysCell separation/fractionationCancerProtein Biochemistry
collection DOAJ
language English
format Article
sources DOAJ
author Giacomo Canesin
Lubica Janovicova
Barbara Wegiel
spellingShingle Giacomo Canesin
Lubica Janovicova
Barbara Wegiel
Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions
STAR Protocols
Cell Biology
Cell-based Assays
Cell separation/fractionation
Cancer
Protein Biochemistry
author_facet Giacomo Canesin
Lubica Janovicova
Barbara Wegiel
author_sort Giacomo Canesin
title Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions
title_short Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions
title_full Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions
title_fullStr Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions
title_full_unstemmed Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions
title_sort measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-06-01
description Summary: Labile heme is present in the cells at very low concentrations, either unbound or loosely bound to molecules, and accessible for signaling as alarmin. Our recent work suggests that extracellular heme can be taken up and detected in the nuclei of cancer cells. Here, we describe the detailed protocol for detection of labile and total heme in prostate cancer cells and its measurement in subcellular compartments in vitro. The protocol can be adapted to be used for other cell types.For complete details on the use and execution of this protocol, please refer to Canesin et al. (2020).
topic Cell Biology
Cell-based Assays
Cell separation/fractionation
Cancer
Protein Biochemistry
url http://www.sciencedirect.com/science/article/pii/S2666166721001982
work_keys_str_mv AT giacomocanesin measurementoflabileandproteinboundhemeinfixedprostatecancercellsandincellularfractions
AT lubicajanovicova measurementoflabileandproteinboundhemeinfixedprostatecancercellsandincellularfractions
AT barbarawegiel measurementoflabileandproteinboundhemeinfixedprostatecancercellsandincellularfractions
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