Protease Activities of Semi-purified Pseudomonas fluorescensin Protein Degradation of Pasteurized Milk at Storage

Protease Activities of Semi-purified Pseudomonas fluorescensin Protein Degradation of Pasteurized Milk at Storage

Protein on stored milks spoiled due to protease activities of Pseudomonas fluorescens. To know protease effect on milks, protease activities of semi-purified P. fluorescens on protein degradation in stored pasteurized milks were detected. Protease was semi-purified by ethanol 70%. Protease activit...

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Bibliographic Details
Main Authors: Tatik Khusniati, Irma Normalia
Format: Article
Language:English
Published: Indonesian Biological Society 2012-10-01
Series:Journal of Biological Researches
Subjects:
protease activities of semi-purified P. fluorescens on protein degradation in stored pasteurized milks were detected. Protease was semi-purified by ethanol 70%. Protease activities were detected by modified Lowry method
protein degradation by formol titration
and protein content by Kjeldahl method. Milk storage' times were conducted on 0 day (4 days before expired date) up to 12 days (8 days after expired date). The results show that the longer the storage times the higher protease activities and protein degradation of milks. At storage 12 days
protease activities on control were 0.2556 Unit/mL (skim) and 0.2116 Unit/mL (whole)
and on inoculated milk (crude) was 2.2044 Unit/mL (skim) and 1.5378 Unit/mL (whole); while on inoculated milk (semi-purified) was 3.5355 Unit/mL (skim) and 1.6778 Unit/mL (whole)
respectively. The decrease of inoculated milk' homogeneous were faster than that of control. Protein degradation on control
inoculated skim milks (crude and semi-purified) on 12 days were 4.48%
7.28% and 7.62%
while that on whole milks were 3.81%
7.28% and 6.04%
respectively. Based on protease
protein degradation and homogeneous
protease
Pseudomonas fluorescens
skim
storage
Pasteurized milk
Protease
Skim
Storage
Online Access:http://berkalahayati.org/journal/51e4e127

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