Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase

Malate dehydrogenase (MDH) is one of the key enzymes for succinic acid (SA) bioproduction. Here, the authors report biochemical and structural analyses of various MDHs to reveal amino acids influencing the specific activity and susceptibility to substrate inhibition, and achieve industrial-level SA...

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Main Authors:	Jung Ho Ahn, Hogyun Seo, Woojin Park, Jihye Seok, Jong An Lee, Won Jun Kim, Gi Bae Kim, Kyung-Jin Kim, Sang Yup Lee
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-04-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-15839-z

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spelling	doaj-c8473773cdfe4e9cbfe7eeeede635aca2021-05-11T08:49:57ZengNature Publishing GroupNature Communications2041-17232020-04-0111111210.1038/s41467-020-15839-zEnhanced succinic acid production by Mannheimia employing optimal malate dehydrogenaseJung Ho Ahn0Hogyun Seo1Woojin Park2Jihye Seok3Jong An Lee4Won Jun Kim5Gi Bae Kim6Kyung-Jin Kim7Sang Yup Lee8Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)School of Life Sciences, KNU Creative BioResearch Group, Kyungpook National UniversitySchool of Life Sciences, KNU Creative BioResearch Group, Kyungpook National UniversitySchool of Life Sciences, KNU Creative BioResearch Group, Kyungpook National UniversityMetabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)School of Life Sciences, KNU Creative BioResearch Group, Kyungpook National UniversityMetabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)Malate dehydrogenase (MDH) is one of the key enzymes for succinic acid (SA) bioproduction. Here, the authors report biochemical and structural analyses of various MDHs to reveal amino acids influencing the specific activity and susceptibility to substrate inhibition, and achieve industrial-level SA production.https://doi.org/10.1038/s41467-020-15839-z
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jung Ho Ahn Hogyun Seo Woojin Park Jihye Seok Jong An Lee Won Jun Kim Gi Bae Kim Kyung-Jin Kim Sang Yup Lee
spellingShingle	Jung Ho Ahn Hogyun Seo Woojin Park Jihye Seok Jong An Lee Won Jun Kim Gi Bae Kim Kyung-Jin Kim Sang Yup Lee Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase Nature Communications
author_facet	Jung Ho Ahn Hogyun Seo Woojin Park Jihye Seok Jong An Lee Won Jun Kim Gi Bae Kim Kyung-Jin Kim Sang Yup Lee
author_sort	Jung Ho Ahn
title	Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase
title_short	Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase
title_full	Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase
title_fullStr	Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase
title_full_unstemmed	Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase
title_sort	enhanced succinic acid production by mannheimia employing optimal malate dehydrogenase
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-04-01
description	Malate dehydrogenase (MDH) is one of the key enzymes for succinic acid (SA) bioproduction. Here, the authors report biochemical and structural analyses of various MDHs to reveal amino acids influencing the specific activity and susceptibility to substrate inhibition, and achieve industrial-level SA production.
url	https://doi.org/10.1038/s41467-020-15839-z
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Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase

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