Exploration of protective effect of Sevoflurane preconditioning on hypoxia reoxygenation injury of myocardial cells in rats and related molecular mechanisms

• Main Authors: Ye-Qiu Li, Zheng-Lan Zhan, Qin-Fang Li
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2016-05-01
• Series: Journal of Hainan Medical University
• Subjects: Sevoflurane; Hypoxia reoxygenation; Mitochondria; Apoptosis; Oxidative stress
• Online Access: http://www.hnykdxxb.com/PDF/201610/3.pdf
• ISSN: 1007-1237; 1007-1237

Objective: To explore the protective effect of Sevoflurane preconditioning on hypoxia reoxygenation injury of myocardial cells in rats and related molecular mechanisms. Methods: Cardiomyocytes of SD rats 1 to 2 days after birth were cultured and divided into NC group (no special treatment), HR group (hypoxia reoxygenation processing) and S group (hypoxia reoxygenation after sevoflurane preconditioning). Myocardial cell injury indexes, mitochondrial pathway apoptosis indexes and oxidative stress indicators were detected. Result: (1) Myocardial injury indexes: myocardial cell apoptosis rate as well as CK, CK-MB, AST and LDH levels in cell culture medium of HR group were significantly higher than those of NC group, and myocardial cell apoptosis rate as well as CK, CK-MB, AST and LDH levels in cell culture medium of S group were significantly lower than those of HR group; (2) mitochondrial pathway apoptosis indexes: Bcl-2, Cx43 and Omi/HtrA2 contents in mitochondria of HR group were lower than those of NC group, and the content of Bax was higher than that of NC group; the contents of cytochrome C and Omi/HtrA2 in cytoplasm were higher than those of NC group, and the content of Cx43 was lower than that of NC group; the contents of Bcl-2, Cx43 and Omi/HtrA2 in mitochondria of S group were higher than those of HR group, and the content of Bax was lower than that of HR group; the contents of cytochrome C and Omi/ HtrA2 in cytoplasm were lower than those of HR group, and the content of Cx43 was higher than that of HR group; (3) oxidative stress indexes: the contents of ROS and MDA in cells of HR group were higher than those of NC group, and the contents of SOD, GSH-Px, VitC and VitE were lower than those of NC group; the contents of ROS and MDA in cells of S group were lower than those of HR group, and the contents of SOD, GSH-Px, VitC and VitE were higher than those of HR group. Conclusion: Sevoflurane preconditioning has protective effect on hypoxia reoxygenation injury of myocardial cells in rats, and its molecular mechanisms include inhibiting mitochondrial pathway apoptosis and relieving oxidative stress.