Crystallisation of Wild-Type and Variant Forms of a Recombinant Plant Enzyme β-D-Glucan Glucohydrolase from Barley (Hordeum vulgare L.) and Preliminary X-ray Analysis

Crystallisation of Wild-Type and Variant Forms of a Recombinant Plant Enzyme β-D-Glucan Glucohydrolase from Barley (Hordeum vulgare L.) and Preliminary X-ray Analysis

Wild-type and variant crystals of a recombinant enzyme β-d-glucan glucohydrolase from barley (Hordeum vulgare L.) were obtained by macroseeding and cross-seeding with microcrystals obtained from native plant protein. Crystals grew to dimensions of up to 500 x 250 x 375 µm at 277 K in the hanging-dro...

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Bibliographic Details
Main Authors: Victor A. Streltsov, Maria Hrmova, James R. Ketudat Cairns, Sukanya Luang
Format: Article
Language:English
Published: MDPI AG 2010-07-01
Series:International Journal of Molecular Sciences
Subjects:
macro- and cross-seeding
wild-type and mutant protein
X-ray diffraction
Online Access:http://www.mdpi.com/1422-0067/11/7/2759/

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