CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation

CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation

Efficient gene targeting in higher plants remains challenging. Here, the authors develop a sequential transformation method for CRISPR/Cas9-mediated gene targeting in Arabidopsis and demonstrate its functionality at five genomic sites in two endogenous loci.

Bibliographic Details
Main Authors: Daisuke Miki, Wenxin Zhang, Wenjie Zeng, Zhengyan Feng, Jian-Kang Zhu
Format: Article
Language:English
Published: Nature Publishing Group 2018-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-04416-0
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spelling doaj-c81bd1b528a94970a1a314b129cdc17f2021-05-11T09:24:35ZengNature Publishing GroupNature Communications2041-17232018-05-01911910.1038/s41467-018-04416-0CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformationDaisuke Miki0Wenxin Zhang1Wenjie Zeng2Zhengyan Feng3Jian-Kang Zhu4Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesShanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesShanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesShanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesShanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesEfficient gene targeting in higher plants remains challenging. Here, the authors develop a sequential transformation method for CRISPR/Cas9-mediated gene targeting in Arabidopsis and demonstrate its functionality at five genomic sites in two endogenous loci.https://doi.org/10.1038/s41467-018-04416-0
collection DOAJ
language English
format Article
sources DOAJ
author Daisuke Miki
Wenxin Zhang
Wenjie Zeng
Zhengyan Feng
Jian-Kang Zhu
spellingShingle Daisuke Miki
Wenxin Zhang
Wenjie Zeng
Zhengyan Feng
Jian-Kang Zhu
CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation
Nature Communications
author_facet Daisuke Miki
Wenxin Zhang
Wenjie Zeng
Zhengyan Feng
Jian-Kang Zhu
author_sort Daisuke Miki
title CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation
title_short CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation
title_full CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation
title_fullStr CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation
title_full_unstemmed CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation
title_sort crispr/cas9-mediated gene targeting in arabidopsis using sequential transformation
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2018-05-01
description Efficient gene targeting in higher plants remains challenging. Here, the authors develop a sequential transformation method for CRISPR/Cas9-mediated gene targeting in Arabidopsis and demonstrate its functionality at five genomic sites in two endogenous loci.
url https://doi.org/10.1038/s41467-018-04416-0
work_keys_str_mv AT daisukemiki crisprcas9mediatedgenetargetinginarabidopsisusingsequentialtransformation
AT wenxinzhang crisprcas9mediatedgenetargetinginarabidopsisusingsequentialtransformation
AT wenjiezeng crisprcas9mediatedgenetargetinginarabidopsisusingsequentialtransformation
AT zhengyanfeng crisprcas9mediatedgenetargetinginarabidopsisusingsequentialtransformation
AT jiankangzhu crisprcas9mediatedgenetargetinginarabidopsisusingsequentialtransformation
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