A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers

A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers

We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the numbe...

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Bibliographic Details
Main Authors: Maríndia Deprá, Lenira Maria Nunes Sepel, Élgion Lucio da Silva Loreto
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2004-01-01
Series:Genetics and Molecular Biology
Subjects:
transgenic Drosophila
piggyBac
GFP
transposable elements
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012
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spelling doaj-c7c2a5b6bf124613b4b9d68b4d24773d2020-11-25T02:22:46ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852004-01-01271707310.1590/S1415-47572004000100012A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markersMaríndia DepráLenira Maria Nunes SepelÉlgion Lucio da Silva LoretoWe describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012transgenic DrosophilapiggyBacGFPtransposable elements
collection DOAJ
language English
format Article
sources DOAJ
author Maríndia Deprá
Lenira Maria Nunes Sepel
Élgion Lucio da Silva Loreto
spellingShingle Maríndia Deprá
Lenira Maria Nunes Sepel
Élgion Lucio da Silva Loreto
A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
Genetics and Molecular Biology
transgenic Drosophila
piggyBac
GFP
transposable elements
author_facet Maríndia Deprá
Lenira Maria Nunes Sepel
Élgion Lucio da Silva Loreto
author_sort Maríndia Deprá
title A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
title_short A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
title_full A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
title_fullStr A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
title_full_unstemmed A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
title_sort low-cost apparatus for transforming drosophila and detecting green fluorescent protein (gfp) genetic markers
publisher Sociedade Brasileira de Genética
series Genetics and Molecular Biology
issn 1415-4757
1678-4685
publishDate 2004-01-01
description We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation.
topic transgenic Drosophila
piggyBac
GFP
transposable elements
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012
work_keys_str_mv AT marindiadepra alowcostapparatusfortransformingdrosophilaanddetectinggreenfluorescentproteingfpgeneticmarkers
AT leniramarianunessepel alowcostapparatusfortransformingdrosophilaanddetectinggreenfluorescentproteingfpgeneticmarkers
AT elgionluciodasilvaloreto alowcostapparatusfortransformingdrosophilaanddetectinggreenfluorescentproteingfpgeneticmarkers
AT marindiadepra lowcostapparatusfortransformingdrosophilaanddetectinggreenfluorescentproteingfpgeneticmarkers
AT leniramarianunessepel lowcostapparatusfortransformingdrosophilaanddetectinggreenfluorescentproteingfpgeneticmarkers
AT elgionluciodasilvaloreto lowcostapparatusfortransformingdrosophilaanddetectinggreenfluorescentproteingfpgeneticmarkers
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