Quantitative analysis of individual bile acids by gas-liquid chromatography: an improved method

The quantitative analysis of individual bile acids by gas-liquid chromatography has been improved by column oven temperature programming and by a new liquid phase, SP-2401. The method is fast; bile acids are well resolved; retention times are reproducible; detector responses are linear and sensitive...

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Main Authors: D C Jones, G W Melchior, M J W Reeves
Format: Article
Language:English
Published: Elsevier 1976-05-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520369844
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spelling doaj-c70834972d2c47c4806aca28b814fc932021-04-24T05:49:45ZengElsevierJournal of Lipid Research0022-22751976-05-01173273277Quantitative analysis of individual bile acids by gas-liquid chromatography: an improved methodD C Jones0G W Melchior1M J W Reeves2The Atherosclerosis Center, Department of Pathology, Bowman Gray School of Medicine, Winston-Salem, North Carolina 27103The Atherosclerosis Center, Department of Pathology, Bowman Gray School of Medicine, Winston-Salem, North Carolina 27103The Atherosclerosis Center, Department of Pathology, Bowman Gray School of Medicine, Winston-Salem, North Carolina 27103The quantitative analysis of individual bile acids by gas-liquid chromatography has been improved by column oven temperature programming and by a new liquid phase, SP-2401. The method is fast; bile acids are well resolved; retention times are reproducible; detector responses are linear and sensitive to 0.1 μg; and there is little adsorption onto the liquid phase. The method has been successfully used for bile, and it has the potential for use on serum.http://www.sciencedirect.com/science/article/pii/S0022227520369844trifluoroacetatesbileSP-2401 liquid phasesquirrel monkey
collection DOAJ
language English
format Article
sources DOAJ
author D C Jones
G W Melchior
M J W Reeves
spellingShingle D C Jones
G W Melchior
M J W Reeves
Quantitative analysis of individual bile acids by gas-liquid chromatography: an improved method
Journal of Lipid Research
trifluoroacetates
bile
SP-2401 liquid phase
squirrel monkey
author_facet D C Jones
G W Melchior
M J W Reeves
author_sort D C Jones
title Quantitative analysis of individual bile acids by gas-liquid chromatography: an improved method
title_short Quantitative analysis of individual bile acids by gas-liquid chromatography: an improved method
title_full Quantitative analysis of individual bile acids by gas-liquid chromatography: an improved method
title_fullStr Quantitative analysis of individual bile acids by gas-liquid chromatography: an improved method
title_full_unstemmed Quantitative analysis of individual bile acids by gas-liquid chromatography: an improved method
title_sort quantitative analysis of individual bile acids by gas-liquid chromatography: an improved method
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1976-05-01
description The quantitative analysis of individual bile acids by gas-liquid chromatography has been improved by column oven temperature programming and by a new liquid phase, SP-2401. The method is fast; bile acids are well resolved; retention times are reproducible; detector responses are linear and sensitive to 0.1 μg; and there is little adsorption onto the liquid phase. The method has been successfully used for bile, and it has the potential for use on serum.
topic trifluoroacetates
bile
SP-2401 liquid phase
squirrel monkey
url http://www.sciencedirect.com/science/article/pii/S0022227520369844
work_keys_str_mv AT dcjones quantitativeanalysisofindividualbileacidsbygasliquidchromatographyanimprovedmethod
AT gwmelchior quantitativeanalysisofindividualbileacidsbygasliquidchromatographyanimprovedmethod
AT mjwreeves quantitativeanalysisofindividualbileacidsbygasliquidchromatographyanimprovedmethod
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