Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model
Qing’e Pills is a Chinese traditional herbal product, which is often used to strengthen muscles and bones in TCM (traditional Chinese Medicine) practice. Its two main component herbs, namely, <i>Cortex Eucommiae</i> and <i>Fructus Psoraleae</i> are both required to...
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doaj-c6d032df19bf4c7182b4fea29c0d3c3e2020-11-25T01:02:25ZengMDPI AGMolecules1420-30492018-12-012312332110.3390/molecules23123321molecules23123321Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell ModelJinlan Lu0Ling Liu1Xingyu Zhu2Li Wu3Zhipeng Chen4Zisheng Xu5Weidong Li6College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaWuhu Pure Sunshine Natural Medicine Company Limited, Wuhu 241000, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaQing’e Pills is a Chinese traditional herbal product, which is often used to strengthen muscles and bones in TCM (traditional Chinese Medicine) practice. Its two main component herbs, namely, <i>Cortex Eucommiae</i> and <i>Fructus Psoraleae</i> are both required to be salt-fried according to TCM theory. We have evaluated the effects of salt-frying treated herbs on Caco-2 cell uptake behavior for those active ingredients of Qing’e Pills. By investigating of various variables, including MTT, temperature, inhibitors, pH, salt concentration and herb processing methods, we tried to clarify whether the salt-processing on herbs was necessary or not. Results showed that, compared to other processing methods, the salt-frying process significantly (<i>p</i> < 0.01) enhanced the absorption of effective components of Qing’e Pills. The way that psoralen, isopsoralen, psoralenoside and geniposide acid entered Caco-2 cells at low concentrations was via passive diffusion. These components were not substrates of P-glycoprotein. It demonstrated that the salt-frying process not only enhanced the concentration of active components in herb extract, but also changed their absorption behaviors. Nevertheless, the mechanism of absorption behavior changing needs to be further investigated.https://www.mdpi.com/1420-3049/23/12/3321Qing’e PillsCaco-2 cellabsorptionsalt-fried process |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jinlan Lu Ling Liu Xingyu Zhu Li Wu Zhipeng Chen Zisheng Xu Weidong Li |
spellingShingle |
Jinlan Lu Ling Liu Xingyu Zhu Li Wu Zhipeng Chen Zisheng Xu Weidong Li Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model Molecules Qing’e Pills Caco-2 cell absorption salt-fried process |
author_facet |
Jinlan Lu Ling Liu Xingyu Zhu Li Wu Zhipeng Chen Zisheng Xu Weidong Li |
author_sort |
Jinlan Lu |
title |
Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model |
title_short |
Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model |
title_full |
Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model |
title_fullStr |
Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model |
title_full_unstemmed |
Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model |
title_sort |
evaluation of the absorption behavior of main component compounds of salt-fried herb ingredients in qing’e pills by using caco-2 cell model |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-12-01 |
description |
Qing’e Pills is a Chinese traditional herbal product, which is often used to strengthen muscles and bones in TCM (traditional Chinese Medicine) practice. Its two main component herbs, namely, <i>Cortex Eucommiae</i> and <i>Fructus Psoraleae</i> are both required to be salt-fried according to TCM theory. We have evaluated the effects of salt-frying treated herbs on Caco-2 cell uptake behavior for those active ingredients of Qing’e Pills. By investigating of various variables, including MTT, temperature, inhibitors, pH, salt concentration and herb processing methods, we tried to clarify whether the salt-processing on herbs was necessary or not. Results showed that, compared to other processing methods, the salt-frying process significantly (<i>p</i> < 0.01) enhanced the absorption of effective components of Qing’e Pills. The way that psoralen, isopsoralen, psoralenoside and geniposide acid entered Caco-2 cells at low concentrations was via passive diffusion. These components were not substrates of P-glycoprotein. It demonstrated that the salt-frying process not only enhanced the concentration of active components in herb extract, but also changed their absorption behaviors. Nevertheless, the mechanism of absorption behavior changing needs to be further investigated. |
topic |
Qing’e Pills Caco-2 cell absorption salt-fried process |
url |
https://www.mdpi.com/1420-3049/23/12/3321 |
work_keys_str_mv |
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