Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model

Qing&#8217;e Pills is a Chinese traditional herbal product, which is often used to strengthen muscles and bones in TCM (traditional Chinese Medicine) practice. Its two main component herbs, namely, <i>Cortex Eucommiae</i> and <i>Fructus Psoraleae</i> are both required to...

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Main Authors: Jinlan Lu, Ling Liu, Xingyu Zhu, Li Wu, Zhipeng Chen, Zisheng Xu, Weidong Li
Format: Article
Language:English
Published: MDPI AG 2018-12-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/23/12/3321
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spelling doaj-c6d032df19bf4c7182b4fea29c0d3c3e2020-11-25T01:02:25ZengMDPI AGMolecules1420-30492018-12-012312332110.3390/molecules23123321molecules23123321Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell ModelJinlan Lu0Ling Liu1Xingyu Zhu2Li Wu3Zhipeng Chen4Zisheng Xu5Weidong Li6College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaWuhu Pure Sunshine Natural Medicine Company Limited, Wuhu 241000, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, ChinaQing&#8217;e Pills is a Chinese traditional herbal product, which is often used to strengthen muscles and bones in TCM (traditional Chinese Medicine) practice. Its two main component herbs, namely, <i>Cortex Eucommiae</i> and <i>Fructus Psoraleae</i> are both required to be salt-fried according to TCM theory. We have evaluated the effects of salt-frying treated herbs on Caco-2 cell uptake behavior for those active ingredients of Qing&#8217;e Pills. By investigating of various variables, including MTT, temperature, inhibitors, pH, salt concentration and herb processing methods, we tried to clarify whether the salt-processing on herbs was necessary or not. Results showed that, compared to other processing methods, the salt-frying process significantly (<i>p</i> &lt; 0.01) enhanced the absorption of effective components of Qing&#8217;e Pills. The way that psoralen, isopsoralen, psoralenoside and geniposide acid entered Caco-2 cells at low concentrations was via passive diffusion. These components were not substrates of P-glycoprotein. It demonstrated that the salt-frying process not only enhanced the concentration of active components in herb extract, but also changed their absorption behaviors. Nevertheless, the mechanism of absorption behavior changing needs to be further investigated.https://www.mdpi.com/1420-3049/23/12/3321Qing’e PillsCaco-2 cellabsorptionsalt-fried process
collection DOAJ
language English
format Article
sources DOAJ
author Jinlan Lu
Ling Liu
Xingyu Zhu
Li Wu
Zhipeng Chen
Zisheng Xu
Weidong Li
spellingShingle Jinlan Lu
Ling Liu
Xingyu Zhu
Li Wu
Zhipeng Chen
Zisheng Xu
Weidong Li
Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model
Molecules
Qing’e Pills
Caco-2 cell
absorption
salt-fried process
author_facet Jinlan Lu
Ling Liu
Xingyu Zhu
Li Wu
Zhipeng Chen
Zisheng Xu
Weidong Li
author_sort Jinlan Lu
title Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model
title_short Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model
title_full Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model
title_fullStr Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model
title_full_unstemmed Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model
title_sort evaluation of the absorption behavior of main component compounds of salt-fried herb ingredients in qing’e pills by using caco-2 cell model
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2018-12-01
description Qing&#8217;e Pills is a Chinese traditional herbal product, which is often used to strengthen muscles and bones in TCM (traditional Chinese Medicine) practice. Its two main component herbs, namely, <i>Cortex Eucommiae</i> and <i>Fructus Psoraleae</i> are both required to be salt-fried according to TCM theory. We have evaluated the effects of salt-frying treated herbs on Caco-2 cell uptake behavior for those active ingredients of Qing&#8217;e Pills. By investigating of various variables, including MTT, temperature, inhibitors, pH, salt concentration and herb processing methods, we tried to clarify whether the salt-processing on herbs was necessary or not. Results showed that, compared to other processing methods, the salt-frying process significantly (<i>p</i> &lt; 0.01) enhanced the absorption of effective components of Qing&#8217;e Pills. The way that psoralen, isopsoralen, psoralenoside and geniposide acid entered Caco-2 cells at low concentrations was via passive diffusion. These components were not substrates of P-glycoprotein. It demonstrated that the salt-frying process not only enhanced the concentration of active components in herb extract, but also changed their absorption behaviors. Nevertheless, the mechanism of absorption behavior changing needs to be further investigated.
topic Qing’e Pills
Caco-2 cell
absorption
salt-fried process
url https://www.mdpi.com/1420-3049/23/12/3321
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AT lingliu evaluationoftheabsorptionbehaviorofmaincomponentcompoundsofsaltfriedherbingredientsinqingepillsbyusingcaco2cellmodel
AT xingyuzhu evaluationoftheabsorptionbehaviorofmaincomponentcompoundsofsaltfriedherbingredientsinqingepillsbyusingcaco2cellmodel
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AT zhipengchen evaluationoftheabsorptionbehaviorofmaincomponentcompoundsofsaltfriedherbingredientsinqingepillsbyusingcaco2cellmodel
AT zishengxu evaluationoftheabsorptionbehaviorofmaincomponentcompoundsofsaltfriedherbingredientsinqingepillsbyusingcaco2cellmodel
AT weidongli evaluationoftheabsorptionbehaviorofmaincomponentcompoundsofsaltfriedherbingredientsinqingepillsbyusingcaco2cellmodel
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