Qualitative microanalysis and estimation of sphingolipid bases
A method is described for the identification and determination of sphingolipid long-chain bases in various animal and plant lipids. Sphingosine and related bases, isolated as a mixture from acid hydrolysates of sphingolipids, are oxidized by sodium metaperiodate and the fatty aldehyde reaction produ...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1959-10-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520390908 |
| Summary: | A method is described for the identification and determination of sphingolipid long-chain bases in various animal and plant lipids. Sphingosine and related bases, isolated as a mixture from acid hydrolysates of sphingolipids, are oxidized by sodium metaperiodate and the fatty aldehyde reaction products are isolated and analyzed by gas-liquid partition chromatography. The records thus obtained reflect the composition of the sphingolipid base fraction. A preliminary survey of various tissues is reported and the types of long-chain base found for each tissue are given. Evidence is presented for the presence of a new long-chain base associatcd with the sphingomyelin fraction of human plasma lipids. |
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| ISSN: | 0022-2275 |