The heat shock protein family gene Hspa1l in male mice is dispensable for fertility

Background Heat shock protein family A member 1 like (Hspa1l) is a member of the 70kD heat shock protein (Hsp70) family. HSPA1L is an ancient, evolutionarily conserved gene with a highly conserved domain structure. The gene is highly abundant and constitutively expressed in the mice testes. However,...

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Main Authors:	Xin Wang, Wenxiu Xie, Yejin Yao, Yunfei Zhu, Jianli Zhou, Yiqiang Cui, Xuejiang Guo, Yan Yuan, Zuomin Zhou, Mingxi Liu
Format:	Article
Language:	English
Published:	PeerJ Inc. 2020-03-01
Series:	PeerJ
Subjects:	HSPA1L Spermatogenesis Male infertility Gene knockout
Online Access:	https://peerj.com/articles/8702.pdf

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record_format	Article
spelling	doaj-c673d075e645458294cb1361b6bdf9e62020-11-25T02:10:02ZengPeerJ Inc.PeerJ2167-83592020-03-018e870210.7717/peerj.8702The heat shock protein family gene Hspa1l in male mice is dispensable for fertilityXin Wang0Wenxiu Xie1Yejin Yao2Yunfei Zhu3Jianli Zhou4Yiqiang Cui5Xuejiang Guo6Yan Yuan7Zuomin Zhou8Mingxi Liu9Department of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaDepartment of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaDepartment of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaDepartment of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaAnimal Core Facility, Nanjing Medical University, Nanjing, Jiangsu, ChinaDepartment of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaDepartment of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaDepartment of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaDepartment of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaDepartment of Histology and Embryology, Nanjing Medical University, Nanjing, ChinaBackground Heat shock protein family A member 1 like (Hspa1l) is a member of the 70kD heat shock protein (Hsp70) family. HSPA1L is an ancient, evolutionarily conserved gene with a highly conserved domain structure. The gene is highly abundant and constitutively expressed in the mice testes. However, the role of Hspa1l in the testes has still not been elucidated. Methods Hspa1l-mutant mice were generated using the CRISPR/Cas9 system. Histological and immunofluorescence staining were used to analyze the phenotypes of testis and epididymis. Apoptotic cells were detected through TUNEL assays. Fertility and sperm motilities were also tested. Quantitative RT-PCR was used for analyzing of candidate genes expression. Heat treatment was used to induce heat stress of the testis. Results We successfully generated Hspa1l knockout mice. Hspa1l-/- mice exhibited normal development and fertility. Further, Hspa1l-/- mice shown no significant difference in spermatogenesis, the number of apoptotic cells in testes epididymal histology, sperm count and sperm motility from Hspa1l+/+ mice. Moreover, heat stress does not exacerbate the cell apoptosis in Hspa1l-/- testes. These results revealed that HSPA1L is not essential for physiological spermatogenesis, nor is it involved in heat-induced stress responses, which provides a basis for further studies.https://peerj.com/articles/8702.pdfHSPA1LSpermatogenesisMale infertilityGene knockout
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Xin Wang Wenxiu Xie Yejin Yao Yunfei Zhu Jianli Zhou Yiqiang Cui Xuejiang Guo Yan Yuan Zuomin Zhou Mingxi Liu
spellingShingle	Xin Wang Wenxiu Xie Yejin Yao Yunfei Zhu Jianli Zhou Yiqiang Cui Xuejiang Guo Yan Yuan Zuomin Zhou Mingxi Liu The heat shock protein family gene Hspa1l in male mice is dispensable for fertility PeerJ HSPA1L Spermatogenesis Male infertility Gene knockout
author_facet	Xin Wang Wenxiu Xie Yejin Yao Yunfei Zhu Jianli Zhou Yiqiang Cui Xuejiang Guo Yan Yuan Zuomin Zhou Mingxi Liu
author_sort	Xin Wang
title	The heat shock protein family gene Hspa1l in male mice is dispensable for fertility
title_short	The heat shock protein family gene Hspa1l in male mice is dispensable for fertility
title_full	The heat shock protein family gene Hspa1l in male mice is dispensable for fertility
title_fullStr	The heat shock protein family gene Hspa1l in male mice is dispensable for fertility
title_full_unstemmed	The heat shock protein family gene Hspa1l in male mice is dispensable for fertility
title_sort	heat shock protein family gene hspa1l in male mice is dispensable for fertility
publisher	PeerJ Inc.
series	PeerJ
issn	2167-8359
publishDate	2020-03-01
description	Background Heat shock protein family A member 1 like (Hspa1l) is a member of the 70kD heat shock protein (Hsp70) family. HSPA1L is an ancient, evolutionarily conserved gene with a highly conserved domain structure. The gene is highly abundant and constitutively expressed in the mice testes. However, the role of Hspa1l in the testes has still not been elucidated. Methods Hspa1l-mutant mice were generated using the CRISPR/Cas9 system. Histological and immunofluorescence staining were used to analyze the phenotypes of testis and epididymis. Apoptotic cells were detected through TUNEL assays. Fertility and sperm motilities were also tested. Quantitative RT-PCR was used for analyzing of candidate genes expression. Heat treatment was used to induce heat stress of the testis. Results We successfully generated Hspa1l knockout mice. Hspa1l-/- mice exhibited normal development and fertility. Further, Hspa1l-/- mice shown no significant difference in spermatogenesis, the number of apoptotic cells in testes epididymal histology, sperm count and sperm motility from Hspa1l+/+ mice. Moreover, heat stress does not exacerbate the cell apoptosis in Hspa1l-/- testes. These results revealed that HSPA1L is not essential for physiological spermatogenesis, nor is it involved in heat-induced stress responses, which provides a basis for further studies.
topic	HSPA1L Spermatogenesis Male infertility Gene knockout
url	https://peerj.com/articles/8702.pdf
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The heat shock protein family gene Hspa1l in male mice is dispensable for fertility

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