MicroRNA-4316 inhibits gastric cancer proliferation and migration via directly targeting VEGF-A

• Main Authors: Haithm Mousa, Menglang Yuan, Xinsheng Zhang, Xiaomeng Li, Abdullah Shopit, Marwan Almoiliqy, Mohammed Alshwmi, Aisha Al-Dherasi, Yue Xu, Yunfei Zuo
• Format: Article
• Language: English
• Published: BMC 2020-02-01
• Series: Cancer Cell International
• Subjects: Gastric cancer (GC); miR-4316; VEGF-A; Proliferation; Metastasis
• Online Access: http://link.springer.com/article/10.1186/s12935-020-1132-3

Abstract Background and aims microRNAs (miRNAs) have been reported to regulate proliferation and migration by down-regulating the expression of target genes. The aims of this study were to investigate whether miR-4316 inhibited proliferation and migration by downregulating vascular endothelial growth factor A (VEGF-A) and its clinical significance in gastric cancer (GC). Methods The clinical tissues of the GC patients for miR-4316 and VEGF-A were detected by qRT-PCR. The protein levels of VEGF-A and c-Met were determined by western blotting. Cell Proliferation, migration, and colony forming assays were conducted to show whether miR-4316 affects proliferation by CCK-8, migration by transwell, wound healing and colony formation assays. The bioinformatic methods and luciferase reporter assay were applied to detect the relationship between miRNA and VEGF-A on its targeting 3-untranslated regions (3-UTRs). CCK-8, colony formation, wound healing, and transwell assay were performed to explore the function of miR-4316. Results The results of qRT-PCR indicated that miR-4316 expression level was significantly downregulated in human GC tissues and GC cell lines compared with their control. miR-4316 inhibited proliferation, migration and colony formation in GC cell lines by reducing VEGF-A. And western blot results indicated that miR-4316 significantly inhibited GC through repressing VEGF-A and c-Met. The investigation of Luciferase assay indicated that VEGF-A is a direct target gene of miR-4316. Conclusions miR-4316 suppressed proliferation and migration of GC through the VEGF-A gene. MiR-4316 acts as a tumor suppressor by targeting VEGF-A and this indicated that MiR-4316 might be a potential therapeutic target for GC.