Detection of Extended-Spectrum ß-lactamases Production by Escherichia coli: A Phenotypic Comparative Study
Resistance to antibiotics is a serious concern and immediate steps are required to crub the resistance gained by microorganisms. Extended spectrum beta- lactamases (ESBLs) producing E.coli (Escherichia coli) is rapidly increasing and making antibiotics difficult to treat around the world. The scre...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2018-12-01
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| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/detection-of-extended-spectrum-s-lactamases-production-by-escherichia-coli-a-phenotypic-comparative-study/ |
| Summary: | Resistance to antibiotics is a serious concern and immediate steps are required to crub the resistance
gained by microorganisms. Extended spectrum beta- lactamases (ESBLs) producing E.coli (Escherichia
coli) is rapidly increasing and making antibiotics difficult to treat around the world. The screening of
ESBL producers among Escherichia coli was done by method Double Disk Synergy Test (DDST), using
ceftazidime and ceftriaxone disc and Combined Disc Test (CD) using ceftazidime-clavulanic acid according
to Clinical and Laboratory Standard Institute (CLSI) protocol. In the DDST method, out of 60 clinical
isolates of Escherichia coli, 22 were found ESBL positive isolates, and among 22 positive isolates 18
strains shows increase in zone of inhibition by both ceftazidime and ceftriaxone disc. The remaining 4
ESBL isolates, 3 of them showed zone of inhibition to ceftazidime and only 1 isolate showed inhibition
by ceftriaxone disc. Similarly, in CD method apart from 22 isolates detected by DDST method it detected
1 more isolate as ESBL producers. Furthermore, the CD method detects inducible beta- lactamase in
III generation of cephalosporin sensitive isolate. Our study showed 22 isolates were found by DDST
method and 23 isolates by CD method. This study results showed the prevalence of ESBL in E. coli were
36.66% by DDST and 38.33% by CD tests and we conclude that combined disk test (CD) is better than
DDST for detection of ESBL. |
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| ISSN: | 0973-7510 2581-690X |