Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder

Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder

Chicken egg yolk is an easily available source for the isolation of sialylglycopeptides (SGP) carrying homogenous biantennary N-glycans. This approach has gained much attention in the last decade since these SGPs can easily be used for the semi-synthesis of glycoconjugates circumventing laborious fu...

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Main Authors: Kathirvel Alagesan, Daniel Kolarich
Format: Article
Language:English
Published: Elsevier 2019-01-01
Series:MethodsX
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016119300949
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spelling doaj-c63c9374cfa941f49741eef3cfa929772020-11-25T02:13:09ZengElsevierMethodsX2215-01612019-01-016773778Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powderKathirvel Alagesan0Daniel Kolarich1Department of Biomolecular Sciences, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany; Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany; Institute for Glycomics, Griffith University, Gold Coast Campus, QLD, 4222, AustraliaDepartment of Biomolecular Sciences, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany; Institute for Glycomics, Griffith University, Gold Coast Campus, QLD, 4222, Australia; ARC Centre for Nanoscale BioPhotonics, Australia; Corresponding author at: Institute for Glycomics, Building G26, Griffith University, Gold Coast Campus, Southport, 4222 QLD, Australia.Chicken egg yolk is an easily available source for the isolation of sialylglycopeptides (SGP) carrying homogenous biantennary N-glycans. This approach has gained much attention in the last decade since these SGPs can easily be used for the semi-synthesis of glycoconjugates circumventing laborious full-synthetic methodologies. Here we report an optimised, significantly shorter (one day instead of five) and environmentally friendly procedure for the mg scale isolation of SGP using commercially available egg yolk powder. A single chromatographic step following chloroform/methanol precipitation of proteins and lipids yielded desired approximately 200 mg SGP from 250 g egg yolk powder within a day. • Environmentally friendly procedure for isolation of sialylglycopeptide from Egg yolk powder. • Reduced the protocol from five days down to one. Method name: Improved strategy for large scale isolation and purification of sialylglycopeptide from egg yolk powder, Keywords: Synthetic glycobiology, Glycopeptide isolation, Egg yolk powderhttp://www.sciencedirect.com/science/article/pii/S2215016119300949
collection DOAJ
language English
format Article
sources DOAJ
author Kathirvel Alagesan
Daniel Kolarich
spellingShingle Kathirvel Alagesan
Daniel Kolarich
Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder
MethodsX
author_facet Kathirvel Alagesan
Daniel Kolarich
author_sort Kathirvel Alagesan
title Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder
title_short Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder
title_full Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder
title_fullStr Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder
title_full_unstemmed Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder
title_sort improved strategy for large scale isolation of sialylglycopeptide (sgp) from egg yolk powder
publisher Elsevier
series MethodsX
issn 2215-0161
publishDate 2019-01-01
description Chicken egg yolk is an easily available source for the isolation of sialylglycopeptides (SGP) carrying homogenous biantennary N-glycans. This approach has gained much attention in the last decade since these SGPs can easily be used for the semi-synthesis of glycoconjugates circumventing laborious full-synthetic methodologies. Here we report an optimised, significantly shorter (one day instead of five) and environmentally friendly procedure for the mg scale isolation of SGP using commercially available egg yolk powder. A single chromatographic step following chloroform/methanol precipitation of proteins and lipids yielded desired approximately 200 mg SGP from 250 g egg yolk powder within a day. • Environmentally friendly procedure for isolation of sialylglycopeptide from Egg yolk powder. • Reduced the protocol from five days down to one. Method name: Improved strategy for large scale isolation and purification of sialylglycopeptide from egg yolk powder, Keywords: Synthetic glycobiology, Glycopeptide isolation, Egg yolk powder
url http://www.sciencedirect.com/science/article/pii/S2215016119300949
work_keys_str_mv AT kathirvelalagesan improvedstrategyforlargescaleisolationofsialylglycopeptidesgpfromeggyolkpowder
AT danielkolarich improvedstrategyforlargescaleisolationofsialylglycopeptidesgpfromeggyolkpowder
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