Examination of Exhaustive Cloning Attempts Reveals that C. elegans piRNAs, Transposons, and Repeat Sequences are Efficiently Cloned in Yeast, but not in Bacteria
Genome sequencing requires insertion of random fragments of the sequenced organism’s DNA into a unicellular host, most often E. coli bacteria. This manipulation was found in the past to be analogous to naturally occurring horizontal gene transfer, and moreover has proved valuable to understanding to...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014-08-01
|
| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fgene.2014.00275/full |