Lentiviral vector transduction of spermatozoa as a tool for the study of early development

Lentiviral vector transduction of spermatozoa as a tool for the study of early development

Spermatozoa and lentiviruses are two of nature’s most efficient gene delivery vehicles. Both can be genetically modified and used independently for the generation of transgenic animals or gene transfer/therapy of inherited disorders. Here we show that mature spermatozoa can be directly transduced wi...

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Main Authors: Anil Chandrashekran, Ihsan Isa, Jayesh Dudhia, Adrian J. Thrasher, Nicholas Dibb, Colin Casimir, Carol Readhead, Robert Winston
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:FEBS Open Bio
Subjects:
Lentiviral vectors
Spermatozoa
Transduction
Development
Transgenics
In vitro fertilisation
Online Access:http://www.sciencedirect.com/science/article/pii/S2211546314000199
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spelling doaj-c54c118ecb3849058ec0c46be9d20dd42020-11-25T03:57:43ZengWileyFEBS Open Bio2211-54632014-01-014C26627510.1016/j.fob.2014.02.008Lentiviral vector transduction of spermatozoa as a tool for the study of early developmentAnil Chandrashekran0Ihsan Isa1Jayesh Dudhia2Adrian J. Thrasher3Nicholas Dibb4Colin Casimir5Carol Readhead6Robert Winston7Department of Surgery and Cancer, Division of Cancer, Imperial College London, Hammersmith Campus, Institute of Reproductive and Developmental Biology (IRDB), Du Cane Road, London W12 0NN, UKDepartment of Surgery and Cancer, Division of Cancer, Imperial College London, Hammersmith Campus, Institute of Reproductive and Developmental Biology (IRDB), Du Cane Road, London W12 0NN, UKDepartment of Clinical Science Services, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UKMolecular Immunology Unit, UCL-Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UKDepartment of Surgery and Cancer, Division of Cancer, Imperial College London, Hammersmith Campus, Institute of Reproductive and Developmental Biology (IRDB), Du Cane Road, London W12 0NN, UKDepartment of Natural Sciences, School of Science & Technology, Middlesex University, The Burroughs, London NW4 4BT, UKBiological Imaging Center, California Institute of Technology, Beckman Institute, 139-74, Pasadena, CA 91135, USADepartment of Surgery and Cancer, Division of Cancer, Imperial College London, Hammersmith Campus, Institute of Reproductive and Developmental Biology (IRDB), Du Cane Road, London W12 0NN, UKSpermatozoa and lentiviruses are two of nature’s most efficient gene delivery vehicles. Both can be genetically modified and used independently for the generation of transgenic animals or gene transfer/therapy of inherited disorders. Here we show that mature spermatozoa can be directly transduced with various pseudotyped lentiviral vectors and used in in vitro fertilisation studies. Lentiviral vectors encoding Green Fluorescent Protein (GFP) were shown to be efficiently processed and expressed in sperm. When these transduced sperm were used in in vitro fertilisation studies, GFP expression was observed in arising blastocysts. This simple technique of directly transducing spermatozoa has potential to be a powerful tool for the study of early and pre-implantation development and could be used as a technique in transgenic development and vertical viral transmission studies.http://www.sciencedirect.com/science/article/pii/S2211546314000199Lentiviral vectorsSpermatozoaTransductionDevelopmentTransgenicsIn vitro fertilisation
collection DOAJ
language English
format Article
sources DOAJ
author Anil Chandrashekran
Ihsan Isa
Jayesh Dudhia
Adrian J. Thrasher
Nicholas Dibb
Colin Casimir
Carol Readhead
Robert Winston
spellingShingle Anil Chandrashekran
Ihsan Isa
Jayesh Dudhia
Adrian J. Thrasher
Nicholas Dibb
Colin Casimir
Carol Readhead
Robert Winston
Lentiviral vector transduction of spermatozoa as a tool for the study of early development
FEBS Open Bio
Lentiviral vectors
Spermatozoa
Transduction
Development
Transgenics
In vitro fertilisation
author_facet Anil Chandrashekran
Ihsan Isa
Jayesh Dudhia
Adrian J. Thrasher
Nicholas Dibb
Colin Casimir
Carol Readhead
Robert Winston
author_sort Anil Chandrashekran
title Lentiviral vector transduction of spermatozoa as a tool for the study of early development
title_short Lentiviral vector transduction of spermatozoa as a tool for the study of early development
title_full Lentiviral vector transduction of spermatozoa as a tool for the study of early development
title_fullStr Lentiviral vector transduction of spermatozoa as a tool for the study of early development
title_full_unstemmed Lentiviral vector transduction of spermatozoa as a tool for the study of early development
title_sort lentiviral vector transduction of spermatozoa as a tool for the study of early development
publisher Wiley
series FEBS Open Bio
issn 2211-5463
publishDate 2014-01-01
description Spermatozoa and lentiviruses are two of nature’s most efficient gene delivery vehicles. Both can be genetically modified and used independently for the generation of transgenic animals or gene transfer/therapy of inherited disorders. Here we show that mature spermatozoa can be directly transduced with various pseudotyped lentiviral vectors and used in in vitro fertilisation studies. Lentiviral vectors encoding Green Fluorescent Protein (GFP) were shown to be efficiently processed and expressed in sperm. When these transduced sperm were used in in vitro fertilisation studies, GFP expression was observed in arising blastocysts. This simple technique of directly transducing spermatozoa has potential to be a powerful tool for the study of early and pre-implantation development and could be used as a technique in transgenic development and vertical viral transmission studies.
topic Lentiviral vectors
Spermatozoa
Transduction
Development
Transgenics
In vitro fertilisation
url http://www.sciencedirect.com/science/article/pii/S2211546314000199
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