Directed evolution of the bacterial endo-β-1,4-glucanase from Streptomyces sp. G12 towards improved catalysts for lignocellulose conversion

Directed evolution of the bacterial endo-β-1,4-glucanase from Streptomyces sp. G12 towards improved catalysts for lignocellulose conversion

Abstract With the aim to develop biocatalysts for enhanced hydrolysis of (hemi)cellulose into monosaccharides, random diversity by directed evolution was introduced in the gene coding for the endo-β-1,4-glucanase from Streptomyces sp. G12 which had been recombinantly expressed in Escherichia coli an...

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Bibliographic Details
Main Authors: Davide Agostino Cecchini, Olimpia Pepe, Anna Pennacchio, Massimo Fagnano, Vincenza Faraco
Format: Article
Language:English
Published: SpringerOpen 2018-05-01
Series:AMB Express
Subjects:
Directed evolution
High-throughput screening
Cellulase
Arundo donax
Saccharification
Online Access:http://link.springer.com/article/10.1186/s13568-018-0602-7

Internet

http://link.springer.com/article/10.1186/s13568-018-0602-7

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