Efficient Improving the Activity and Enantioselectivity of Candida rugosa Lipase for the Resolution of Naproxen by Enzyme Immobilization on MCM-41 Mesoporous Molecular Sieve

• Main Authors: Ying Chen, Yi Xu, Xiao-mei Wu
• Format: Article
• Language: English
• Published: Bulgarian Academy of Sciences 2015-09-01
• Series: International Journal Bioautomation
• Subjects: MCM-41; Immobilization; Lipase; Enantioselectivity; Naproxen; Stability
• Online Access: http://www.biomed.bas.bg/bioautomation/2015/vol_19.3/files/19.3_05.pdf

Lipase from Candida rogusa was immobilized on MCM-41 mesoporous molecular sieves in a trapped aqueous-organic biphase system for the resolution of racemic naproxen methyl ester. It was interesting that the activity and enantioselectivity of the immobilized lipase were improved significantly relative to the free enzyme. The proportion of water (ml)/support (g) has a dramatic influence on the activity and enantioselectivity of lipase immobilized onto MCM-41 molecular sieves. It was also found that the activity of immobilized lipase was more sensitive to pH value and temperature than the free one. Higher pH value will increase the activity but decrease the enantioselectivity of the immobilized lipase. The enantioselectivity of the immobilized lipase was not altered significantly within the range of tested temperature. The immobilized lipase can be reused for at least 8 batches without significant lose of activity with the aid of methanotrophic bacteria to eliminate the methanol produced during the resolution process.