Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules

• Main Authors: Gamal Rayan, Jean-Erik Guet, Nicolas Taulier, Frederic Pincet, Wladimir Urbach
• Format: Article
• Language: English
• Published: MDPI AG 2010-06-01
• Series: Sensors
• Subjects: biomembranes; Fluorescence Recovery after Photobleaching (FRAP); diffusion; biomolecular interactions
• Online Access: http://www.mdpi.com/1424-8220/10/6/5927/

This review examines some recent applications of fluorescence recovery after photobleaching (FRAP) to biopolymers, while mainly focusing on membrane protein studies. Initially, we discuss the lateral diffusion of membrane proteins, as measured by FRAP. Then, we talk about the use of FRAP to probe interactions between membrane proteins by obtaining fundamental information such as geometry and stoichiometry of the interacting complex. Afterwards, we discuss some applications of FRAP at the cellular level as well as the level of organisms. We conclude by comparing diffusion coefficients obtained by FRAP and several other alternative methods.