Downregulation of signal transduction and STAT3 expression exacerbates oxidative stress mediated by NLRP3 inflammasome
Activated nucleotide binding to the oligonucleotide receptor protein 3 (NLRP3) inflammasome is possibly involved in the pathogenesis of Alzheimer's disease through oxidative stress and neurogenic inflammation. Low expression of the signal transducer and activator of transcription 3 (STAT3) gene...
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Wolters Kluwer Medknow Publications
2018-01-01
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doaj-c51805c969e2494fb9362b4e858a1d8f2020-11-25T03:49:14ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742018-01-0113122147215510.4103/1673-5374.241470Downregulation of signal transduction and STAT3 expression exacerbates oxidative stress mediated by NLRP3 inflammasomeHua BaiQi-Fang ZhangJuan-Juan DuanDe-Jun YuLi-Jie LiuActivated nucleotide binding to the oligonucleotide receptor protein 3 (NLRP3) inflammasome is possibly involved in the pathogenesis of Alzheimer's disease through oxidative stress and neurogenic inflammation. Low expression of the signal transducer and activator of transcription 3 (STAT3) gene may promote the occurrence of neurodegenerative diseases to some extent. To clarify the roles of the NLRP3 inflammasome and STAT3 expression in oxidative stress, (1) SHSY5Y cells were incubated with 1 mM H2O2 to induce oxidative stress injury, and the expression of human-cell-specific signal transduction, STAT3-shRNA silencing signal transduction and STAT3 were detected. Cells were pretreated with Ca2+ chelator BAPATA-AM (0.1 mM) for 30 minutes as a control. (2) Western blot assay was used to analyze the expression of caspase-1, NLRP3, signal transduction and STAT3. Enzyme-linked immunosorbent assay was used to analyze interleukin-1β levels. Flow cytometry was carried out to calculate the number of apoptotic cells. We found that H2O2 treatment activated NLRP3 inflammasomes and decreased phosphorylation of signal transduction and STAT3 serine 727. BAPTA-AM pretreatment abolished the H2O2-induced activation of NLRP3 inflammasomes, caspase-1 expression, interleukin-1β expression and apoptosis in SHSY5Y cells, and had no effect in cells with downregulated STAT3 expression by RNAi. The findings suggest that downregulation of signal transduction and STAT3 expression may enhance the oxidative stress mediated by NLRP3, which may not depend on the Ca2+ signaling pathway.http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=12;spage=2147;epage=2155;aulast=Bainerve regeneration; signal transducer and activator of transcription 3; calcium; caspase-1; nucleotide binding to the oligonucleotide receptor protein 3; inflammasome; hydrogen peroxide; Alzheimer′s disease; shRNA; SHSY5Y cells; neural regeneration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hua Bai Qi-Fang Zhang Juan-Juan Duan De-Jun Yu Li-Jie Liu |
spellingShingle |
Hua Bai Qi-Fang Zhang Juan-Juan Duan De-Jun Yu Li-Jie Liu Downregulation of signal transduction and STAT3 expression exacerbates oxidative stress mediated by NLRP3 inflammasome Neural Regeneration Research nerve regeneration; signal transducer and activator of transcription 3; calcium; caspase-1; nucleotide binding to the oligonucleotide receptor protein 3; inflammasome; hydrogen peroxide; Alzheimer′s disease; shRNA; SHSY5Y cells; neural regeneration |
author_facet |
Hua Bai Qi-Fang Zhang Juan-Juan Duan De-Jun Yu Li-Jie Liu |
author_sort |
Hua Bai |
title |
Downregulation of signal transduction and STAT3 expression exacerbates oxidative stress mediated by NLRP3 inflammasome |
title_short |
Downregulation of signal transduction and STAT3 expression exacerbates oxidative stress mediated by NLRP3 inflammasome |
title_full |
Downregulation of signal transduction and STAT3 expression exacerbates oxidative stress mediated by NLRP3 inflammasome |
title_fullStr |
Downregulation of signal transduction and STAT3 expression exacerbates oxidative stress mediated by NLRP3 inflammasome |
title_full_unstemmed |
Downregulation of signal transduction and STAT3 expression exacerbates oxidative stress mediated by NLRP3 inflammasome |
title_sort |
downregulation of signal transduction and stat3 expression exacerbates oxidative stress mediated by nlrp3 inflammasome |
publisher |
Wolters Kluwer Medknow Publications |
series |
Neural Regeneration Research |
issn |
1673-5374 |
publishDate |
2018-01-01 |
description |
Activated nucleotide binding to the oligonucleotide receptor protein 3 (NLRP3) inflammasome is possibly involved in the pathogenesis of Alzheimer's disease through oxidative stress and neurogenic inflammation. Low expression of the signal transducer and activator of transcription 3 (STAT3) gene may promote the occurrence of neurodegenerative diseases to some extent. To clarify the roles of the NLRP3 inflammasome and STAT3 expression in oxidative stress, (1) SHSY5Y cells were incubated with 1 mM H2O2 to induce oxidative stress injury, and the expression of human-cell-specific signal transduction, STAT3-shRNA silencing signal transduction and STAT3 were detected. Cells were pretreated with Ca2+ chelator BAPATA-AM (0.1 mM) for 30 minutes as a control. (2) Western blot assay was used to analyze the expression of caspase-1, NLRP3, signal transduction and STAT3. Enzyme-linked immunosorbent assay was used to analyze interleukin-1β levels. Flow cytometry was carried out to calculate the number of apoptotic cells. We found that H2O2 treatment activated NLRP3 inflammasomes and decreased phosphorylation of signal transduction and STAT3 serine 727. BAPTA-AM pretreatment abolished the H2O2-induced activation of NLRP3 inflammasomes, caspase-1 expression, interleukin-1β expression and apoptosis in SHSY5Y cells, and had no effect in cells with downregulated STAT3 expression by RNAi. The findings suggest that downregulation of signal transduction and STAT3 expression may enhance the oxidative stress mediated by NLRP3, which may not depend on the Ca2+ signaling pathway. |
topic |
nerve regeneration; signal transducer and activator of transcription 3; calcium; caspase-1; nucleotide binding to the oligonucleotide receptor protein 3; inflammasome; hydrogen peroxide; Alzheimer′s disease; shRNA; SHSY5Y cells; neural regeneration |
url |
http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=12;spage=2147;epage=2155;aulast=Bai |
work_keys_str_mv |
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