A role for Phospholipase D in <it>Drosophila </it>embryonic cellularization

• Main Authors: Choi Seok-Yong, Altshuller Yelena M, Olive Virginie, Redina Olga, Crosby Juan, Yun Suyong, Janssens Hilde, LaLonde Mary, Du Guangwei, Gergen J Peter, Frohman Michael A
• Format: Article
• Language: English
• Published: BMC 2006-12-01
• Series: BMC Developmental Biology
• Online Access: http://www.biomedcentral.com/1471-213X/6/60

<p>Abstract</p> <p>Background</p> <p>Cellularization of the <it>Drosophila </it>embryo is an unusually synchronous form of cytokinesis in which polarized membrane extension proceeds in part through incorporation of new membrane via fusion of apically-translocated Golgi-derived vesicles.</p> <p>Results</p> <p>We describe here involvement of the signaling enzyme Phospholipase D (Pld) in regulation of this developmental step. Functional analysis using gene targeting revealed that cellularization is hindered by the loss of Pld, resulting frequently in early embryonic developmental arrest. Mechanistically, chronic Pld deficiency causes abnormal Golgi structure and secretory vesicle trafficking.</p> <p>Conclusion</p> <p>Our results suggest that Pld functions to promote trafficking of Golgi-derived fusion-competent vesicles during cellularization.</p>