Is Total Serum Nitrite and Nitrate (NOx) Level in Dengue Patients a Potential Prognostic Marker of Dengue Hemorrhagic Fever?

• Main Authors: Maheshi Mapalagamage, Shiroma Handunnetti, Gayani Premawansa, Sharmila Thillainathan, Tharanga Fernando, Karunayokiny Kanapathippillai, Rajitha Wickremasinghe, Aruna Dharshan De Silva, Sunil Premawansa
• Format: Article
• Language: English
• Published: Hindawi Limited 2018-01-01
• Series: Disease Markers
• Online Access: http://dx.doi.org/10.1155/2018/5328681

Potential use of total nitrite plus nitrate (NOx) and nitrite (NO2−) separately as surrogate markers for serum nitric oxide in severe dengue and their longitudinal changes along with the progression of infection was studied. Deproteinized sera from confirmed dengue fever (DF, n=145) and dengue hemorrhagic fever (DHF, n=74) patients on admission—A, critical—C, discharge—D, and convalescence—CON stages and from age-gender matched healthy individuals (HC, n=77) were taken to assess NO2− and NOx levels using Griess and modified Griess assays. Serum NOx in DHFA was significantly lower compared to DFA (p<0.001). HC had the lowest NOx and NO2− compared to all patient categories (p<0.001) except NO2− in DF-CON and DHF-CON and NOx in DHF-CON. Serum NOx and NO2− in DHF patients admitted on fever day 3 (DHFA-3) was significantly lower compared to DFA-3 (p<0.05). Cut-off values of 4.46 μM for NOx (91.3% sensitivity and 80.1% specificity) and 1.25 μM for NO2− (75.0% sensitivity and 73.3% specificity) were obtained for day 3 of fever. Serum NOx may be used as potential prognostic marker of DHF in patients presenting with DF in the early stage (on day 3 of fever) of the disease.