Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases

Pinoresinol–lariciresinol reductases (PLRs) are enzymes involved in the lignan biosynthesis. Here, crystal structures of three PLRs in the apo, substrate-bound and product-bound states, and accompanying mutagenesis provide insight into PLRs catalytic mechanism and suggest a strategy for PLR engineer...

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Main Authors:	Ying Xiao, Kai Shao, Jingwen Zhou, Lian Wang, Xueqi Ma, Di Wu, Yingbo Yang, Junfeng Chen, Jingxian Feng, Shi Qiu, Zongyou Lv, Lei Zhang, Peng Zhang, Wansheng Chen
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-05-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-021-23095-y

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spelling	doaj-c447762f36564f00bfa424a474de6a372021-05-16T11:09:46ZengNature Publishing GroupNature Communications2041-17232021-05-0112111110.1038/s41467-021-23095-yStructure-based engineering of substrate specificity for pinoresinol-lariciresinol reductasesYing Xiao0Kai Shao1Jingwen Zhou2Lian Wang3Xueqi Ma4Di Wu5Yingbo Yang6Junfeng Chen7Jingxian Feng8Shi Qiu9Zongyou Lv10Lei Zhang11Peng Zhang12Wansheng Chen13Research and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese MedicineNational Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesNational Engineering Laboratory for Cereal Fermentation Technology and School of Biotechnology, Jiangnan UniversityNational Engineering Laboratory for Cereal Fermentation Technology and School of Biotechnology, Jiangnan UniversityResearch and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese MedicineNational Engineering Laboratory for Cereal Fermentation Technology and School of Biotechnology, Jiangnan UniversityResearch and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese MedicineResearch and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese MedicineResearch and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese MedicineResearch and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese MedicineResearch and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese MedicineDepartment of Pharmaceutical Botany, School of Pharmacy, Naval Medical University (Second Military Medical University)National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of SciencesResearch and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese MedicinePinoresinol–lariciresinol reductases (PLRs) are enzymes involved in the lignan biosynthesis. Here, crystal structures of three PLRs in the apo, substrate-bound and product-bound states, and accompanying mutagenesis provide insight into PLRs catalytic mechanism and suggest a strategy for PLR engineering.https://doi.org/10.1038/s41467-021-23095-y
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Ying Xiao Kai Shao Jingwen Zhou Lian Wang Xueqi Ma Di Wu Yingbo Yang Junfeng Chen Jingxian Feng Shi Qiu Zongyou Lv Lei Zhang Peng Zhang Wansheng Chen
spellingShingle	Ying Xiao Kai Shao Jingwen Zhou Lian Wang Xueqi Ma Di Wu Yingbo Yang Junfeng Chen Jingxian Feng Shi Qiu Zongyou Lv Lei Zhang Peng Zhang Wansheng Chen Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases Nature Communications
author_facet	Ying Xiao Kai Shao Jingwen Zhou Lian Wang Xueqi Ma Di Wu Yingbo Yang Junfeng Chen Jingxian Feng Shi Qiu Zongyou Lv Lei Zhang Peng Zhang Wansheng Chen
author_sort	Ying Xiao
title	Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
title_short	Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
title_full	Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
title_fullStr	Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
title_full_unstemmed	Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
title_sort	structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2021-05-01
description	Pinoresinol–lariciresinol reductases (PLRs) are enzymes involved in the lignan biosynthesis. Here, crystal structures of three PLRs in the apo, substrate-bound and product-bound states, and accompanying mutagenesis provide insight into PLRs catalytic mechanism and suggest a strategy for PLR engineering.
url	https://doi.org/10.1038/s41467-021-23095-y
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Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases

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