Deciphering the Infectious Process of <i>Colletotrichum lupini </i>in Lupin through Transcriptomic and Proteomic Analysis

• Main Authors: Guillaume Dubrulle, Adeline Picot, Stéphanie Madec, Erwan Corre, Audrey Pawtowski, Riccardo Baroncelli, Michel Zivy, Thierry Balliau, Gaétan Le Floch, Flora Pensec
• Format: Article
• Language: English
• Published: MDPI AG 2020-10-01
• Series: Microorganisms
• Subjects: anthracnose disease; transcriptome; proteome; pathogenicity factors
• Online Access: https://www.mdpi.com/2076-2607/8/10/1621

The fungal phytopathogen <i>Colletotrichum lupini</i> is responsible for lupin anthracnose, resulting in significant yield losses worldwide. The molecular mechanisms underlying this infectious process are yet to be elucidated. This study proposes to evaluate <i>C. lupini </i>gene expression and protein synthesis during lupin infection, using, respectively, an RNAseq-based transcriptomic approach and a mass spectrometry-based proteomic approach. Patterns of differentially-expressed genes in planta were evaluated from 24 to 84 hours post-inoculation, and compared to in vitro cultures. A total of 897 differentially-expressed genes were identified from <i>C. lupini</i> during interaction with white lupin, of which 520 genes were predicted to have a putative function, including carbohydrate active enzyme, effector, protease or transporter-encoding genes, commonly described as pathogenicity factors for other <i>Colletotrichum</i> species during plant infection, and 377 hypothetical proteins. Simultaneously, a total of 304 proteins produced during the interaction were identified and quantified by mass spectrometry. Taken together, the results highlight that the dynamics of symptoms, gene expression and protein synthesis shared similarities to those of hemibiotrophic pathogens. In addition, a few genes with unknown or poorly-described functions were found to be specifically associated with the early or late stages of infection, suggesting that they may be of importance for pathogenicity. This study, conducted for the first time on a species belonging to the <i>Colletotrichum acutatum</i> species complex, presents an opportunity to deepen functional analyses of the genes involved in the pathogenicity of <i>Colletotrichum</i> spp. during the onset of plant infection.