KIM-1 Mediates High Glucose-Induced Autophagy and Apoptosis in Renal Tubular Epithelial Cells

Background/Aim: To investigate the role of kidney injury molecular 1 (KIM-1) in high glucose-induced autophagy and apoptosis in renal tubular epithelial cells. Methods: Human renal tubular epithelial cells (HK2) were treated with normal glucose (NG, D -glucose 5.6 mmol/L), high glucose (HG, 30 mmol/...

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Main Authors: Rong Gou, Juntong Chen, Shifeng Sheng, Ruiqiang Wang, Yudong Fang, Zijun Yang, Liuwei Wang, Lin Tang
Format: Article
Language:English
Published: Cell Physiol Biochem Press GmbH & Co KG 2016-06-01
Series:Cellular Physiology and Biochemistry
Subjects:
Online Access:http://www.karger.com/Article/FullText/445598
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spelling doaj-c41edc722436458e847e74be6e713a352020-11-25T02:40:29ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782016-06-013862479248810.1159/000445598445598KIM-1 Mediates High Glucose-Induced Autophagy and Apoptosis in Renal Tubular Epithelial CellsRong GouJuntong ChenShifeng ShengRuiqiang WangYudong FangZijun YangLiuwei WangLin TangBackground/Aim: To investigate the role of kidney injury molecular 1 (KIM-1) in high glucose-induced autophagy and apoptosis in renal tubular epithelial cells. Methods: Human renal tubular epithelial cells (HK2) were treated with normal glucose (NG, D -glucose 5.6 mmol/L), high glucose (HG, 30 mmol/L), high osmotic (HO, D-glucose 5.6 mmol/L + D-mannitol 24.4 mmol/L), HG + KIM-1 siRNA, HG + siRNA control. The expressions of KIM-1 and microtubule-associated protein 1 light chain 3II (LC3II) were measured by western blot as well as real time PCR; the number of autophagosome was detected by electron microscopy; and the level of apoptosis was analyzed by flow cytometry. Results: In the HG group, the expressions of KIM-1 and LC3II were increased markedly, which was accompanied by more autophagosome and higher level of apoptosis compared with NG group. Silencing of KIM-1 by siRNA inhibited the increases in the levels of LC3II, autophagosome and apoptosis. Conclusion: KIM-1 may mediate high glucose-induced autophagy and apoptosis in renal tubular epithelial cells.http://www.karger.com/Article/FullText/445598LC3IIDiabetic nephropathyAutophagosomeRenal fibrosis
collection DOAJ
language English
format Article
sources DOAJ
author Rong Gou
Juntong Chen
Shifeng Sheng
Ruiqiang Wang
Yudong Fang
Zijun Yang
Liuwei Wang
Lin Tang
spellingShingle Rong Gou
Juntong Chen
Shifeng Sheng
Ruiqiang Wang
Yudong Fang
Zijun Yang
Liuwei Wang
Lin Tang
KIM-1 Mediates High Glucose-Induced Autophagy and Apoptosis in Renal Tubular Epithelial Cells
Cellular Physiology and Biochemistry
LC3II
Diabetic nephropathy
Autophagosome
Renal fibrosis
author_facet Rong Gou
Juntong Chen
Shifeng Sheng
Ruiqiang Wang
Yudong Fang
Zijun Yang
Liuwei Wang
Lin Tang
author_sort Rong Gou
title KIM-1 Mediates High Glucose-Induced Autophagy and Apoptosis in Renal Tubular Epithelial Cells
title_short KIM-1 Mediates High Glucose-Induced Autophagy and Apoptosis in Renal Tubular Epithelial Cells
title_full KIM-1 Mediates High Glucose-Induced Autophagy and Apoptosis in Renal Tubular Epithelial Cells
title_fullStr KIM-1 Mediates High Glucose-Induced Autophagy and Apoptosis in Renal Tubular Epithelial Cells
title_full_unstemmed KIM-1 Mediates High Glucose-Induced Autophagy and Apoptosis in Renal Tubular Epithelial Cells
title_sort kim-1 mediates high glucose-induced autophagy and apoptosis in renal tubular epithelial cells
publisher Cell Physiol Biochem Press GmbH & Co KG
series Cellular Physiology and Biochemistry
issn 1015-8987
1421-9778
publishDate 2016-06-01
description Background/Aim: To investigate the role of kidney injury molecular 1 (KIM-1) in high glucose-induced autophagy and apoptosis in renal tubular epithelial cells. Methods: Human renal tubular epithelial cells (HK2) were treated with normal glucose (NG, D -glucose 5.6 mmol/L), high glucose (HG, 30 mmol/L), high osmotic (HO, D-glucose 5.6 mmol/L + D-mannitol 24.4 mmol/L), HG + KIM-1 siRNA, HG + siRNA control. The expressions of KIM-1 and microtubule-associated protein 1 light chain 3II (LC3II) were measured by western blot as well as real time PCR; the number of autophagosome was detected by electron microscopy; and the level of apoptosis was analyzed by flow cytometry. Results: In the HG group, the expressions of KIM-1 and LC3II were increased markedly, which was accompanied by more autophagosome and higher level of apoptosis compared with NG group. Silencing of KIM-1 by siRNA inhibited the increases in the levels of LC3II, autophagosome and apoptosis. Conclusion: KIM-1 may mediate high glucose-induced autophagy and apoptosis in renal tubular epithelial cells.
topic LC3II
Diabetic nephropathy
Autophagosome
Renal fibrosis
url http://www.karger.com/Article/FullText/445598
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