Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells

• Main Authors: Roshni S. Kalkur, Andrew C. Ballast, Ashley R. Triplett, Kathrin Spendier
• Format: Article
• Language: English
• Published: PeerJ Inc. 2014-09-01
• Series: PeerJ
• Subjects: RBL-2H3 cells; Flow cytometry; Deuterium oxide; Single-particle tracking; TIRF microscopy; Microtubule-dependent vesicle transport
• Online Access: https://peerj.com/articles/553.pdf

For the first time we show the effects of deuterium oxide on cell growth and vesicle transport in rat basophilic leukemia (RBL-2H3) cells. RBL-2H3 cells cultured with 15 moles/L deuterium showed decreased cell growth which was attributed to cells not doubling their DNA content. Experimental observations also showed an increase in vesicle speed for cells cultured in deuterium oxide. This increase in vesicle speed was not observed in deuterium oxide cultures treated with a microtubule-destabilizing drug, suggesting that deuterium oxide affects microtubule-dependent vesicle transport.