Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells

Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells

For the first time we show the effects of deuterium oxide on cell growth and vesicle transport in rat basophilic leukemia (RBL-2H3) cells. RBL-2H3 cells cultured with 15 moles/L deuterium showed decreased cell growth which was attributed to cells not doubling their DNA content. Experimental observat...

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Bibliographic Details
Main Authors: Roshni S. Kalkur, Andrew C. Ballast, Ashley R. Triplett, Kathrin Spendier
Format: Article
Language:English
Published: PeerJ Inc. 2014-09-01
Series:PeerJ
Subjects:
RBL-2H3 cells
Flow cytometry
Deuterium oxide
Single-particle tracking
TIRF microscopy
Microtubule-dependent vesicle transport
Online Access:https://peerj.com/articles/553.pdf
Description
Summary:For the first time we show the effects of deuterium oxide on cell growth and vesicle transport in rat basophilic leukemia (RBL-2H3) cells. RBL-2H3 cells cultured with 15 moles/L deuterium showed decreased cell growth which was attributed to cells not doubling their DNA content. Experimental observations also showed an increase in vesicle speed for cells cultured in deuterium oxide. This increase in vesicle speed was not observed in deuterium oxide cultures treated with a microtubule-destabilizing drug, suggesting that deuterium oxide affects microtubule-dependent vesicle transport.
ISSN:2167-8359

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