Assessment of genetic diversity in maize (Zea mays L.) inbred lines on the basis of soluble seed proteins using SDS-PAGE

• Main Author: Amit Kumar Dixit, Anshuman Singh*, Jai Prakash Shahi, Prabhat Kumar and Dhairyashil Madhukar Langade
• Format: Article
• Language: English
• Published: Indian Society of Plant Breeders 2015-03-01
• Series: Electronic Journal of Plant Breeding
• Subjects: Maize; Genetic diversity; soluble seed protein; SDS-PAGE
• Online Access: https://sites.google.com/site/ejpb2014/vol-6-1/EJPB_V6_N1_264_268.pdf?attredirects=0&d=1
• ISSN: 0975-928X

In the present investigation 25 genotypes of maize collected from different sources were characterized for the soluble seed proteins using sodium dodecyl sulphate polyacrylamide gelelectrophoresis (SDS-PAGE). The total protein bands were separated on 12% polyacrylamide gel using standard protocols. Highest number of bands observed was 14 in inbred HUZQPM-1.Highest coefficient of similarity matrix was0.69whereas lowest was 0.04. The dendrogram prepared by using UPGMA clustering method separated the whole genotypes into two main groups; I and II. On the basis of present investigation, it is observed that there is low variability present among quality protein maize inbreds.