A Sensitive and Accurate Recombinase Polymerase Amplification Assay for Detection of the Primary Bacterial Pathogens Causing Bovine Respiratory Disease

A Sensitive and Accurate Recombinase Polymerase Amplification Assay for Detection of the Primary Bacterial Pathogens Causing Bovine Respiratory Disease

Rapid and accurate diagnosis of bovine respiratory disease (BRD) presents a substantial challenge to the North American cattle industry. Here we utilize recombinase polymerase amplification (RPA), a fast and sensitive isothermal DNA-based technology for the detection of four BRD pathogens (Mannheimi...

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Bibliographic Details
Main Authors: Cheyenne C. Conrad, Rana K. Daher, Kim Stanford, Kingsley K. Amoako, Maurice Boissinot, Michel G. Bergeron, Trevor Alexander, Shaun Cook, Brenda Ralston, Rahat Zaheer, Yan D. Niu, Tim McAllister
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-04-01
Series:Frontiers in Veterinary Science
Subjects:
recombinase polymerase amplification
bovine respiratory disease
antimicrobial resistance
integrative conjugative element
competitive internal amplification
Online Access:https://www.frontiersin.org/article/10.3389/fvets.2020.00208/full

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