Macro and micro spectroscopy parameters of cancerous and healthy gastrointestinal tissues

• Main Authors: Tsanislava Genova, Ekaterina Borisova, Oksana Semyachkina-Glushkovskaya, Dmitry Gorin, Daniil Bratashov, Ivan Terziev
• Format: Article
• Language: English
• Published: Samara National Research University 2017-12-01
• Series: Journal of Biomedical Photonics & Engineering
• Subjects: Autofluorescence; gastrointestinal tissues; colon carcinoma; confocal fluorescent microscopy (CFM)
• Online Access: http://jbpe.ssau.ru/index.php/JBPE/article/view/3264

New more sensitive diagnostic modalities, for either analysis of biopsy tissue samples or on site, in vivo microscopy tissue examination, emerge to aid the detection and accurate diagnosis of gastrointestinal cancer. One of the most intensively investigated techniques is the fluorescence spectroscopy approach. We present our investigation of the detailed macro and micro fluorescence characteristics of ex-vivo fresh and fixed tissue samples. The fluorescence spectra of unprocessed cancerous and healthy gastrointestinal (GIT) tissue, obtained with two approaches fluorescence and synchronous fluorescence spectroscopy were compared with microspectroscopy performed with laser scanning confocal fluorescence microscopy (LSCFM) system in Lambda-scan regime over fixed tissue samples. The evaluated optical macro- and micro- spectroscopy characteristics are presented and disscused in order to improve the current understanding of the origin of autofluorescence contrast between healthy and cancerous tissues and its application for improvement of the existing diagnostic modalities.