Screening of uric acid-lowering active components of corn silk polysaccharide and its targeted improvement on renal excretory dysfunction in hyperuricemia mice

• Main Authors: Xizhu Wang, Liyan Yuan, Zhijie Bao, Baoshang Fu, Pengfei Jiang, Tiecheng Ma, Songyi Lin
• Format: Article
• Language: English
• Published: Elsevier 2021-11-01
• Series: Journal of Functional Foods
• Subjects: Neutral corn silk polysaccharides; Acid corn silk polysaccharides; Structure-activity relationships; Uric acid, hyperuricemia; Xanthine oxidase
• Online Access: http://www.sciencedirect.com/science/article/pii/S1756464621003479

To screen the in vitro uric acid-lowering activity of the neutral polysaccharides (NCSP) and acid polysaccharides (ACSP) isolated from the corn silk were evaluated. Furthermore, preliminary structure-activity relationships for them were investigated. The molecular weight (Mw) of NCSP and ACSP were 74.1 and 109.4 kDa, respectively. NCSP consisted of mannose (Man), glucuronic acid (GlcA), glucose (Glc), galactose (Gal), and arabinose (Ara), in addition to these monosaccharides, the ACSP contains galactose acid (GalA). NMR spectroscopy showed that the NCSP main chain consisted of 1,4,6)-α-D-Glcp, 1,4)-α-D-Glcp, 1,3)-β-D-Galp, and 1,3,5)-β-D-Manp. NCSP exhibited higher activity in anti-hyperuricemia (HUA). The results indicated that serum uric acid (UA) and liver xanthine oxidase (XO) activities levels decreased by 45.71% and 22.4% in the NCSP groups, respectively. In addition, NCSP could significantly improve the kidney damage and promote uric acid excretion. These findings provide evidence in support of NCSP as a potential dietary supplement for improving HUA.