| Summary: | Lactobacillus plantarum CS and Micrococcus luteus CS were isolated from developed fermented milled-mixed corn-soybean wastes-meal in a previous study. In other to confirm the isolates as probiotics, several assays were determined including bile resistance, bile de-conjugation, haemolytic and antimicrobial tests. Their optimum pH, temperature and time-course were also assayed. Lactobacillus plantarum CS and Micrococcus luteus CS had their respective optimal growths within 18 h and 24 h at pH of 5.6 and 6.0 and temperature optima at 37 °C and 31 °C. It was only L. plantarum CS that demonstrated significant (P < 0.05) in-vitro probiotic potential, as it inhibited the indicator pathogens, viz; Staphylococcus aureus, Escherichia coli, Salmonella typhi and Shigella dysentriae, deconjugated bile up to a concentration of 0.3% and had no haemolytic activity. However, M. luteus CS was able to resist 0.2–2% bile concentration and was non- haemolytic as demonstrated by L. plantarum. Generally, their bile resistance potentials improved with increase in cell mass. The results obtained conveyed the information that only L. plantarum CS had strong probiotic potential and its presence in the developed fermented milled corn-soybean wastes suggests that the meal can serve as functional probiotic food. Keywords: Assessment, Fermented meal, L. plantarum CS, M. luteus CS, Probiotics, Properties
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