Co-Administration of Aluminium Hydroxide Nanoparticles and Protective Antigen Domain 4 Encapsulated Non-Ionic Surfactant Vesicles Show Enhanced Immune Response and Superior Protection against Anthrax

Aluminium salts have been the adjuvant of choice in more than 100 licensed vaccines. Here, we have studied the synergistic effect of aluminium hydroxide nanoparticles (AH np) and non-ionic surfactant-based vesicles (NISV) in modulating the immune response against protective antigen domain 4 (D4) of...

Full description

Bibliographic Details
Main Authors: Himanshu Gogoi, Rajesh Mani, Anshu Malik, Parveen Sehrawat, Rakesh Bhatnagar
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:Vaccines
Subjects:
Online Access:https://www.mdpi.com/2076-393X/8/4/571
id doaj-c354f9bf42b343ecb539d30aa8bfba33
record_format Article
spelling doaj-c354f9bf42b343ecb539d30aa8bfba332020-11-25T03:58:23ZengMDPI AGVaccines2076-393X2020-10-01857157110.3390/vaccines8040571Co-Administration of Aluminium Hydroxide Nanoparticles and Protective Antigen Domain 4 Encapsulated Non-Ionic Surfactant Vesicles Show Enhanced Immune Response and Superior Protection against AnthraxHimanshu Gogoi0Rajesh Mani1Anshu Malik2Parveen Sehrawat3Rakesh Bhatnagar4Laboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, IndiaLaboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, IndiaLaboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, IndiaLaboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, IndiaLaboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, IndiaAluminium salts have been the adjuvant of choice in more than 100 licensed vaccines. Here, we have studied the synergistic effect of aluminium hydroxide nanoparticles (AH np) and non-ionic surfactant-based vesicles (NISV) in modulating the immune response against protective antigen domain 4 (D4) of <i>Bacillus anthracis</i>. NISV was prepared from Span 60 and cholesterol, while AH np was prepared from aluminium chloride and sodium hydroxide. AH np was co-administered with NISV encapsulating D4 (NISV-D4) to formulate AHnp/NISV-D4. The antigen-specific immune response of AHnp/NISV-D4 was compared with that of commercial alhydrogel (alhy) co-administered with NISV-D4 (alhydrogel/NISV-D4), NISV-D4, AHnp/D4, and alhydrogel/D4. Co-administration of NISV-D4 with AH np greatly improved the D4-specific antibody titer as compared to the control groups. Based on IgG isotyping and ex vivo cytokine analysis, AHnp/NISV-D4 generated a balanced Th1/Th2 response. Furthermore, AH np/NISV-D4 showed superior protection against anthrax spore challenge in comparison to other groups. Thus, we demonstrate the possibility of developing a novel combinatorial nanoformulation capable of augmenting both humoral and cellular response, paving the way for adjuvant research.https://www.mdpi.com/2076-393X/8/4/571<i>Bacillus anthracis</i>protective antigen domain 4aluminium hydroxide nanoparticlesnon-ionic surfactant vesiclesnanoformulation
collection DOAJ
language English
format Article
sources DOAJ
author Himanshu Gogoi
Rajesh Mani
Anshu Malik
Parveen Sehrawat
Rakesh Bhatnagar
spellingShingle Himanshu Gogoi
Rajesh Mani
Anshu Malik
Parveen Sehrawat
Rakesh Bhatnagar
Co-Administration of Aluminium Hydroxide Nanoparticles and Protective Antigen Domain 4 Encapsulated Non-Ionic Surfactant Vesicles Show Enhanced Immune Response and Superior Protection against Anthrax
Vaccines
<i>Bacillus anthracis</i>
protective antigen domain 4
aluminium hydroxide nanoparticles
non-ionic surfactant vesicles
nanoformulation
author_facet Himanshu Gogoi
Rajesh Mani
Anshu Malik
Parveen Sehrawat
Rakesh Bhatnagar
author_sort Himanshu Gogoi
title Co-Administration of Aluminium Hydroxide Nanoparticles and Protective Antigen Domain 4 Encapsulated Non-Ionic Surfactant Vesicles Show Enhanced Immune Response and Superior Protection against Anthrax
title_short Co-Administration of Aluminium Hydroxide Nanoparticles and Protective Antigen Domain 4 Encapsulated Non-Ionic Surfactant Vesicles Show Enhanced Immune Response and Superior Protection against Anthrax
title_full Co-Administration of Aluminium Hydroxide Nanoparticles and Protective Antigen Domain 4 Encapsulated Non-Ionic Surfactant Vesicles Show Enhanced Immune Response and Superior Protection against Anthrax
title_fullStr Co-Administration of Aluminium Hydroxide Nanoparticles and Protective Antigen Domain 4 Encapsulated Non-Ionic Surfactant Vesicles Show Enhanced Immune Response and Superior Protection against Anthrax
title_full_unstemmed Co-Administration of Aluminium Hydroxide Nanoparticles and Protective Antigen Domain 4 Encapsulated Non-Ionic Surfactant Vesicles Show Enhanced Immune Response and Superior Protection against Anthrax
title_sort co-administration of aluminium hydroxide nanoparticles and protective antigen domain 4 encapsulated non-ionic surfactant vesicles show enhanced immune response and superior protection against anthrax
publisher MDPI AG
series Vaccines
issn 2076-393X
publishDate 2020-10-01
description Aluminium salts have been the adjuvant of choice in more than 100 licensed vaccines. Here, we have studied the synergistic effect of aluminium hydroxide nanoparticles (AH np) and non-ionic surfactant-based vesicles (NISV) in modulating the immune response against protective antigen domain 4 (D4) of <i>Bacillus anthracis</i>. NISV was prepared from Span 60 and cholesterol, while AH np was prepared from aluminium chloride and sodium hydroxide. AH np was co-administered with NISV encapsulating D4 (NISV-D4) to formulate AHnp/NISV-D4. The antigen-specific immune response of AHnp/NISV-D4 was compared with that of commercial alhydrogel (alhy) co-administered with NISV-D4 (alhydrogel/NISV-D4), NISV-D4, AHnp/D4, and alhydrogel/D4. Co-administration of NISV-D4 with AH np greatly improved the D4-specific antibody titer as compared to the control groups. Based on IgG isotyping and ex vivo cytokine analysis, AHnp/NISV-D4 generated a balanced Th1/Th2 response. Furthermore, AH np/NISV-D4 showed superior protection against anthrax spore challenge in comparison to other groups. Thus, we demonstrate the possibility of developing a novel combinatorial nanoformulation capable of augmenting both humoral and cellular response, paving the way for adjuvant research.
topic <i>Bacillus anthracis</i>
protective antigen domain 4
aluminium hydroxide nanoparticles
non-ionic surfactant vesicles
nanoformulation
url https://www.mdpi.com/2076-393X/8/4/571
work_keys_str_mv AT himanshugogoi coadministrationofaluminiumhydroxidenanoparticlesandprotectiveantigendomain4encapsulatednonionicsurfactantvesiclesshowenhancedimmuneresponseandsuperiorprotectionagainstanthrax
AT rajeshmani coadministrationofaluminiumhydroxidenanoparticlesandprotectiveantigendomain4encapsulatednonionicsurfactantvesiclesshowenhancedimmuneresponseandsuperiorprotectionagainstanthrax
AT anshumalik coadministrationofaluminiumhydroxidenanoparticlesandprotectiveantigendomain4encapsulatednonionicsurfactantvesiclesshowenhancedimmuneresponseandsuperiorprotectionagainstanthrax
AT parveensehrawat coadministrationofaluminiumhydroxidenanoparticlesandprotectiveantigendomain4encapsulatednonionicsurfactantvesiclesshowenhancedimmuneresponseandsuperiorprotectionagainstanthrax
AT rakeshbhatnagar coadministrationofaluminiumhydroxidenanoparticlesandprotectiveantigendomain4encapsulatednonionicsurfactantvesiclesshowenhancedimmuneresponseandsuperiorprotectionagainstanthrax
_version_ 1724457529938804736