Nanometre-Scale Visualization of Chemical Parameter Changes by T<sub>1</sub>-Weighted ODMR Imaging Using a Fluorescent Nanodiamond

The dynamics of physical parameters in cells is strongly related to life phenomena; thus, a method to monitor and visualize them on a single-organelle scale would be useful to reveal unknown biological processes. We demonstrate real-time nanometre-scale T<sub>1</sub>-weighted imaging usi...

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Main Authors:	Takahiro Fujisaku, Ryuji Igarashi, Masahiro Shirakawa
Format:	Article
Language:	English
Published:	MDPI AG 2020-08-01
Series:	Chemosensors
Subjects:	nitrogen-vacancy centres fluorescence imaging nanoscale quantum biosensors quantum life science
Online Access:	https://www.mdpi.com/2227-9040/8/3/68

id	doaj-c351fac7b694444da0c96efcf134d0ed
record_format	Article
spelling	doaj-c351fac7b694444da0c96efcf134d0ed2020-11-25T03:17:39ZengMDPI AGChemosensors2227-90402020-08-018686810.3390/chemosensors8030068Nanometre-Scale Visualization of Chemical Parameter Changes by T<sub>1</sub>-Weighted ODMR Imaging Using a Fluorescent NanodiamondTakahiro Fujisaku0Ryuji Igarashi1Masahiro Shirakawa2Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Nishikyo-Ku, Kyoto 615-8510, JapanInstitute for Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, Anagawa 4-9-1, Inage-ku, Chiba 263-8555, JapanDepartment of Molecular Engineering, Graduate School of Engineering, Kyoto University, Nishikyo-Ku, Kyoto 615-8510, JapanThe dynamics of physical parameters in cells is strongly related to life phenomena; thus, a method to monitor and visualize them on a single-organelle scale would be useful to reveal unknown biological processes. We demonstrate real-time nanometre-scale T<sub>1</sub>-weighted imaging using a fluorescent nanodiamond. We explored optically detected magnetic resonance (ODMR) contrast at various values of interval laser pulse (τ), showing that sufficient contrast is obtained by appropriate selection of τ. By this method, we visualized nanometre-scale pH changes using a functionalized nanodiamond whose T<sub>1</sub> has a dependence on pH conditions.https://www.mdpi.com/2227-9040/8/3/68nitrogen-vacancy centresfluorescence imagingnanoscale quantum biosensorsquantum life science
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Takahiro Fujisaku Ryuji Igarashi Masahiro Shirakawa
spellingShingle	Takahiro Fujisaku Ryuji Igarashi Masahiro Shirakawa Nanometre-Scale Visualization of Chemical Parameter Changes by T<sub>1</sub>-Weighted ODMR Imaging Using a Fluorescent Nanodiamond Chemosensors nitrogen-vacancy centres fluorescence imaging nanoscale quantum biosensors quantum life science
author_facet	Takahiro Fujisaku Ryuji Igarashi Masahiro Shirakawa
author_sort	Takahiro Fujisaku
title	Nanometre-Scale Visualization of Chemical Parameter Changes by T<sub>1</sub>-Weighted ODMR Imaging Using a Fluorescent Nanodiamond
title_short	Nanometre-Scale Visualization of Chemical Parameter Changes by T<sub>1</sub>-Weighted ODMR Imaging Using a Fluorescent Nanodiamond
title_full	Nanometre-Scale Visualization of Chemical Parameter Changes by T<sub>1</sub>-Weighted ODMR Imaging Using a Fluorescent Nanodiamond
title_fullStr	Nanometre-Scale Visualization of Chemical Parameter Changes by T<sub>1</sub>-Weighted ODMR Imaging Using a Fluorescent Nanodiamond
title_full_unstemmed	Nanometre-Scale Visualization of Chemical Parameter Changes by T<sub>1</sub>-Weighted ODMR Imaging Using a Fluorescent Nanodiamond
title_sort	nanometre-scale visualization of chemical parameter changes by t<sub>1</sub>-weighted odmr imaging using a fluorescent nanodiamond
publisher	MDPI AG
series	Chemosensors
issn	2227-9040
publishDate	2020-08-01
description	The dynamics of physical parameters in cells is strongly related to life phenomena; thus, a method to monitor and visualize them on a single-organelle scale would be useful to reveal unknown biological processes. We demonstrate real-time nanometre-scale T<sub>1</sub>-weighted imaging using a fluorescent nanodiamond. We explored optically detected magnetic resonance (ODMR) contrast at various values of interval laser pulse (τ), showing that sufficient contrast is obtained by appropriate selection of τ. By this method, we visualized nanometre-scale pH changes using a functionalized nanodiamond whose T<sub>1</sub> has a dependence on pH conditions.
topic	nitrogen-vacancy centres fluorescence imaging nanoscale quantum biosensors quantum life science
url	https://www.mdpi.com/2227-9040/8/3/68
work_keys_str_mv	AT takahirofujisaku nanometrescalevisualizationofchemicalparameterchangesbytsub1subweightedodmrimagingusingafluorescentnanodiamond AT ryujiigarashi nanometrescalevisualizationofchemicalparameterchangesbytsub1subweightedodmrimagingusingafluorescentnanodiamond AT masahiroshirakawa nanometrescalevisualizationofchemicalparameterchangesbytsub1subweightedodmrimagingusingafluorescentnanodiamond
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Nanometre-Scale Visualization of Chemical Parameter Changes by T<sub>1</sub>-Weighted ODMR Imaging Using a Fluorescent Nanodiamond

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