Optimization and Comparison of Different Methods and Factors for Efficient Transformation of Brucella abortus RB51strain

Background: The development of protective vaccines for Brucella spp. has been hampered by the difficulty in transformation of Brucella cells with foreign DNA for genetic manipulation. It seems that the formation of Brucella spheroplasts would increase the efficiency of transformation. The aim of thi...

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Bibliographic Details
Main Authors: Azam Gheibi, Hossein Khanahmad, Gholam Ali Kardar, Maryam Boshtam, Sassan Rezaie, Bahram Kazemi, Mohammad Reza Khorramizadeh
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2019-01-01
Series:Advanced Biomedical Research
Subjects:
Online Access:http://www.advbiores.net/article.asp?issn=2277-9175;year=2019;volume=8;issue=1;spage=37;epage=37;aulast=Gheibi