Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry

Quantitative mass spectrometry enables the proteome-wide assessment of biomolecular binding affinities. While previous approaches mainly focused on protein–small molecule interactions, the authors here present a method to probe protein–DNA and protein–nucleosome binding affinities at proteome scale....

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Main Authors: Matthew M. Makowski, Cathrin Gräwe, Benjamin M. Foster, Nhuong V. Nguyen, Till Bartke, Michiel Vermeulen
Format: Article
Language:English
Published: Nature Publishing Group 2018-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-04084-0
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spelling doaj-c2e2ac02d50d492280b29ec96a5b844c2021-05-11T10:22:58ZengNature Publishing GroupNature Communications2041-17232018-04-019111010.1038/s41467-018-04084-0Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometryMatthew M. Makowski0Cathrin Gräwe1Benjamin M. Foster2Nhuong V. Nguyen3Till Bartke4Michiel Vermeulen5Department of Molecular Biology, Radboud Institute for Molecular Life Sciences, Radboud UniversityDepartment of Molecular Biology, Radboud Institute for Molecular Life Sciences, Radboud UniversityInstitute of Functional Epigenetics, Helmholtz Zentrum MünchenMRC London Institute of Medical Sciences (LMS)Institute of Functional Epigenetics, Helmholtz Zentrum MünchenDepartment of Molecular Biology, Radboud Institute for Molecular Life Sciences, Radboud UniversityQuantitative mass spectrometry enables the proteome-wide assessment of biomolecular binding affinities. While previous approaches mainly focused on protein–small molecule interactions, the authors here present a method to probe protein–DNA and protein–nucleosome binding affinities at proteome scale.https://doi.org/10.1038/s41467-018-04084-0
collection DOAJ
language English
format Article
sources DOAJ
author Matthew M. Makowski
Cathrin Gräwe
Benjamin M. Foster
Nhuong V. Nguyen
Till Bartke
Michiel Vermeulen
spellingShingle Matthew M. Makowski
Cathrin Gräwe
Benjamin M. Foster
Nhuong V. Nguyen
Till Bartke
Michiel Vermeulen
Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry
Nature Communications
author_facet Matthew M. Makowski
Cathrin Gräwe
Benjamin M. Foster
Nhuong V. Nguyen
Till Bartke
Michiel Vermeulen
author_sort Matthew M. Makowski
title Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry
title_short Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry
title_full Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry
title_fullStr Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry
title_full_unstemmed Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry
title_sort global profiling of protein–dna and protein–nucleosome binding affinities using quantitative mass spectrometry
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2018-04-01
description Quantitative mass spectrometry enables the proteome-wide assessment of biomolecular binding affinities. While previous approaches mainly focused on protein–small molecule interactions, the authors here present a method to probe protein–DNA and protein–nucleosome binding affinities at proteome scale.
url https://doi.org/10.1038/s41467-018-04084-0
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