Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry
Quantitative mass spectrometry enables the proteome-wide assessment of biomolecular binding affinities. While previous approaches mainly focused on protein–small molecule interactions, the authors here present a method to probe protein–DNA and protein–nucleosome binding affinities at proteome scale....
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2018-04-01
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Online Access: | https://doi.org/10.1038/s41467-018-04084-0 |
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doaj-c2e2ac02d50d492280b29ec96a5b844c2021-05-11T10:22:58ZengNature Publishing GroupNature Communications2041-17232018-04-019111010.1038/s41467-018-04084-0Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometryMatthew M. Makowski0Cathrin Gräwe1Benjamin M. Foster2Nhuong V. Nguyen3Till Bartke4Michiel Vermeulen5Department of Molecular Biology, Radboud Institute for Molecular Life Sciences, Radboud UniversityDepartment of Molecular Biology, Radboud Institute for Molecular Life Sciences, Radboud UniversityInstitute of Functional Epigenetics, Helmholtz Zentrum MünchenMRC London Institute of Medical Sciences (LMS)Institute of Functional Epigenetics, Helmholtz Zentrum MünchenDepartment of Molecular Biology, Radboud Institute for Molecular Life Sciences, Radboud UniversityQuantitative mass spectrometry enables the proteome-wide assessment of biomolecular binding affinities. While previous approaches mainly focused on protein–small molecule interactions, the authors here present a method to probe protein–DNA and protein–nucleosome binding affinities at proteome scale.https://doi.org/10.1038/s41467-018-04084-0 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Matthew M. Makowski Cathrin Gräwe Benjamin M. Foster Nhuong V. Nguyen Till Bartke Michiel Vermeulen |
spellingShingle |
Matthew M. Makowski Cathrin Gräwe Benjamin M. Foster Nhuong V. Nguyen Till Bartke Michiel Vermeulen Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry Nature Communications |
author_facet |
Matthew M. Makowski Cathrin Gräwe Benjamin M. Foster Nhuong V. Nguyen Till Bartke Michiel Vermeulen |
author_sort |
Matthew M. Makowski |
title |
Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry |
title_short |
Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry |
title_full |
Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry |
title_fullStr |
Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry |
title_full_unstemmed |
Global profiling of protein–DNA and protein–nucleosome binding affinities using quantitative mass spectrometry |
title_sort |
global profiling of protein–dna and protein–nucleosome binding affinities using quantitative mass spectrometry |
publisher |
Nature Publishing Group |
series |
Nature Communications |
issn |
2041-1723 |
publishDate |
2018-04-01 |
description |
Quantitative mass spectrometry enables the proteome-wide assessment of biomolecular binding affinities. While previous approaches mainly focused on protein–small molecule interactions, the authors here present a method to probe protein–DNA and protein–nucleosome binding affinities at proteome scale. |
url |
https://doi.org/10.1038/s41467-018-04084-0 |
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