| Summary: | In this paper, Lactocin C-M2(C-M2) was used together with a new non-thermal technology, non-thermal plasma sterilization (NTPS), to inactive the putrefactive bacteria <i>Morganella</i> sp. wf-1 isolated from aquatic foods. The mechanism underlining the action mode of C-M2 and NTPS was investigated, revealing that the bacteriocin and NTPS had synergistic effects on the disinfection of <i>Morganella</i> sp. wf-1. Compared with the bacteria cells treated by only C-M2 or NTPS, the plasmolysis of cells treated by C-M2 and NTPS was to a larger extent. Moreover, the cell permeability and the contents of UV-absorbing compounds and K<sup>+</sup> released from the intra-cells was significantly higher for the C-M2 + NTPS treated cells than the others (<i>p</i> < 0.05), and conversely was the SFA/UFA ratio (<i>p</i> < 0.05). The results on DNA damage showed that, 8-hydroxy-2′-deoxyguanosine(8-OHdG) content in C-M2 + NTPS treated cells was approximately 7 -fold and 2.5-fold greater than those in the C-M2- and NTPS-treated cells, respectively, indicating furthermore the eventual rupture of <i>Morganella</i> sp. wf-1 cells. The results showed the potential of the application of the bacteriocin and NTPS in the food industry.
|
|---|
