Multi-Modal Mass Spectrometric Imaging of Uveal Melanoma
Matrix assisted laser desorption ionisation mass spectrometry imaging (MALDI-MSI), was used to obtain images of lipids and metabolite distribution in formalin fixed and embedded in paraffin (FFPE) whole eye sections containing primary uveal melanomas (UM). Using this technique, it was possible to ob...
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MDPI AG
2021-08-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/8/560 |
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doaj-c2c20a9c9a4f4e419a1da98f2f7b985b2021-08-26T14:03:58ZengMDPI AGMetabolites2218-19892021-08-011156056010.3390/metabo11080560Multi-Modal Mass Spectrometric Imaging of Uveal MelanomaLaura M. Cole0Joshua Handley1Emmanuelle Claude2Catherine J. Duckett3Hardeep S. Mudhar4Karen Sisley5Malcolm R. Clench6Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UKCentre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UKWaters Corporation, Wilmslow SK9 4AX, UKCentre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UKNSOPS, Department of Histopathology, E-Floor, Royal Hallamshire Hospital, Sheffield S10 2JF, UKDepartment of Oncology and Metabolism, The Medical School, University of Sheffield, Sheffield S10 2RX, UKCentre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UKMatrix assisted laser desorption ionisation mass spectrometry imaging (MALDI-MSI), was used to obtain images of lipids and metabolite distribution in formalin fixed and embedded in paraffin (FFPE) whole eye sections containing primary uveal melanomas (UM). Using this technique, it was possible to obtain images of lysophosphatidylcholine (LPC) type lipid distribution that highlighted the tumour regions. Laser ablation inductively coupled plasma mass spectrometry images (LA-ICP-MS) performed on UM sections showed increases in copper within the tumour periphery and intratumoural zinc in tissue from patients with poor prognosis. These preliminary data indicate that multi-modal MSI has the potential to provide insights into the role of trace metals and cancer metastasis.https://www.mdpi.com/2218-1989/11/8/560uveal melanoma mass spectrometry imagingmatrix assisted laser desorption ionisationlaser ablation inductively coupled plasmacancer progressiongenetic biomarkersheterogeneity |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Laura M. Cole Joshua Handley Emmanuelle Claude Catherine J. Duckett Hardeep S. Mudhar Karen Sisley Malcolm R. Clench |
| spellingShingle |
Laura M. Cole Joshua Handley Emmanuelle Claude Catherine J. Duckett Hardeep S. Mudhar Karen Sisley Malcolm R. Clench Multi-Modal Mass Spectrometric Imaging of Uveal Melanoma Metabolites uveal melanoma mass spectrometry imaging matrix assisted laser desorption ionisation laser ablation inductively coupled plasma cancer progression genetic biomarkers heterogeneity |
| author_facet |
Laura M. Cole Joshua Handley Emmanuelle Claude Catherine J. Duckett Hardeep S. Mudhar Karen Sisley Malcolm R. Clench |
| author_sort |
Laura M. Cole |
| title |
Multi-Modal Mass Spectrometric Imaging of Uveal Melanoma |
| title_short |
Multi-Modal Mass Spectrometric Imaging of Uveal Melanoma |
| title_full |
Multi-Modal Mass Spectrometric Imaging of Uveal Melanoma |
| title_fullStr |
Multi-Modal Mass Spectrometric Imaging of Uveal Melanoma |
| title_full_unstemmed |
Multi-Modal Mass Spectrometric Imaging of Uveal Melanoma |
| title_sort |
multi-modal mass spectrometric imaging of uveal melanoma |
| publisher |
MDPI AG |
| series |
Metabolites |
| issn |
2218-1989 |
| publishDate |
2021-08-01 |
| description |
Matrix assisted laser desorption ionisation mass spectrometry imaging (MALDI-MSI), was used to obtain images of lipids and metabolite distribution in formalin fixed and embedded in paraffin (FFPE) whole eye sections containing primary uveal melanomas (UM). Using this technique, it was possible to obtain images of lysophosphatidylcholine (LPC) type lipid distribution that highlighted the tumour regions. Laser ablation inductively coupled plasma mass spectrometry images (LA-ICP-MS) performed on UM sections showed increases in copper within the tumour periphery and intratumoural zinc in tissue from patients with poor prognosis. These preliminary data indicate that multi-modal MSI has the potential to provide insights into the role of trace metals and cancer metastasis. |
| topic |
uveal melanoma mass spectrometry imaging matrix assisted laser desorption ionisation laser ablation inductively coupled plasma cancer progression genetic biomarkers heterogeneity |
| url |
https://www.mdpi.com/2218-1989/11/8/560 |
| work_keys_str_mv |
AT lauramcole multimodalmassspectrometricimagingofuvealmelanoma AT joshuahandley multimodalmassspectrometricimagingofuvealmelanoma AT emmanuelleclaude multimodalmassspectrometricimagingofuvealmelanoma AT catherinejduckett multimodalmassspectrometricimagingofuvealmelanoma AT hardeepsmudhar multimodalmassspectrometricimagingofuvealmelanoma AT karensisley multimodalmassspectrometricimagingofuvealmelanoma AT malcolmrclench multimodalmassspectrometricimagingofuvealmelanoma |
| _version_ |
1721191610893467648 |