A new culture medium for recovering the agents of Cryptococcosis from environmental sources

A new culture medium for recovering the agents of Cryptococcosis from environmental sources

The isolation of Cryptococcosis agents from environmental samples may be difficult due to the presence of groups of fast-growing fungi. We propose a new culture medium based on a modification of Dichloran Rose-Bengal Chloramphenicol Medium (DRBCm) to detect colonies of Cryptococcus neoformans. Our r...

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Bibliographic Details
Main Authors: Dulcilena de Matos Castro e Silva, Dayane C.S. Santos, Sandra R.B.S. Pukinskas, Julia T.U. Oshida, Lidiane Oliveira, Anderson F. Carvallho, Márcia S.C. Melhem
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia 2015-06-01
Series:Brazilian Journal of Microbiology
Subjects:
Cryptococcus
isolation techniques
DRBC
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200355&lng=en&tlng=en
Description
Summary:The isolation of Cryptococcosis agents from environmental samples may be difficult due to the presence of groups of fast-growing fungi. We propose a new culture medium based on a modification of Dichloran Rose-Bengal Chloramphenicol Medium (DRBCm) to detect colonies of Cryptococcus neoformans. Our results indicate that DRBCm is superior to the classical Bird Seed Agar in its ability to detect colonies of C. neoformans.
ISSN:1678-4405

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