Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.)
Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube. In this study, we combined existing simple sequence repeat (SSR) markers (nuclear microsatellites) in the nov...
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2016-01-01
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Series: | Silva Fennica |
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doaj-c20288d584704ea180317bdf7b907a602020-11-25T02:26:48ZengFinnish Society of Forest ScienceSilva Fennica2242-40752016-01-0150510.14214/sf.1674Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.)Ćelepirović, NevenkaKarija Vlahović, MonikaDounavi, AikateriniIvanković, Mladen Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube. In this study, we combined existing simple sequence repeat (SSR) markers (nuclear microsatellites) in the novel combination of multiplex PCR to study the population genetics of common walnut from Croatia. From twenty one tested SSR markers, eleven produced satisfactory results in one multiplex PCR. Population genetic results achieved from 15 samples of Croatian common walnut showed moderate genetic variability (average value: He 0.473; Ho 0.568). Our multiplex PCR allowed cost effective work concerning chemicals, plastic ware, device, and working time producing optimal results. The optimized multiplex PCR represented the best combination of eleven SSR primers for genotyping common walnut in a single PCR reaction.https://www.silvafennica.fi/article/1674 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ćelepirović, Nevenka Karija Vlahović, Monika Dounavi, Aikaterini Ivanković, Mladen |
spellingShingle |
Ćelepirović, Nevenka Karija Vlahović, Monika Dounavi, Aikaterini Ivanković, Mladen Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.) Silva Fennica |
author_facet |
Ćelepirović, Nevenka Karija Vlahović, Monika Dounavi, Aikaterini Ivanković, Mladen |
author_sort |
Ćelepirović, Nevenka |
title |
Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.) |
title_short |
Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.) |
title_full |
Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.) |
title_fullStr |
Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.) |
title_full_unstemmed |
Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.) |
title_sort |
optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>juglans regia</i> l.) |
publisher |
Finnish Society of Forest Science |
series |
Silva Fennica |
issn |
2242-4075 |
publishDate |
2016-01-01 |
description |
Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube. In this study, we combined existing simple sequence repeat (SSR) markers (nuclear microsatellites) in the novel combination of multiplex PCR to study the population genetics of common walnut from Croatia. From twenty one tested SSR markers, eleven produced satisfactory results in one multiplex PCR. Population genetic results achieved from 15 samples of Croatian common walnut showed moderate genetic variability (average value: He 0.473; Ho 0.568). Our multiplex PCR allowed cost effective work concerning chemicals, plastic ware, device, and working time producing optimal results. The optimized multiplex PCR represented the best combination of eleven SSR primers for genotyping common walnut in a single PCR reaction. |
url |
https://www.silvafennica.fi/article/1674 |
work_keys_str_mv |
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