Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.)

Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube. In this study, we combined existing simple sequence repeat (SSR) markers (nuclear microsatellites) in the nov...

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Main Authors: Ćelepirović, Nevenka, Karija Vlahović, Monika, Dounavi, Aikaterini, Ivanković, Mladen
Format: Article
Language:English
Published: Finnish Society of Forest Science 2016-01-01
Series:Silva Fennica
Online Access:https://www.silvafennica.fi/article/1674
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spelling doaj-c20288d584704ea180317bdf7b907a602020-11-25T02:26:48ZengFinnish Society of Forest ScienceSilva Fennica2242-40752016-01-0150510.14214/sf.1674Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.)Ćelepirović, NevenkaKarija Vlahović, MonikaDounavi, AikateriniIvanković, Mladen Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube. In this study, we combined existing simple sequence repeat (SSR) markers (nuclear microsatellites) in the novel combination of multiplex PCR to study the population genetics of common walnut from Croatia. From twenty one tested SSR markers, eleven produced satisfactory results in one multiplex PCR. Population genetic results achieved from 15 samples of Croatian common walnut showed moderate genetic variability (average value: He 0.473; Ho 0.568). Our multiplex PCR allowed cost effective work concerning chemicals, plastic ware, device, and working time producing optimal results. The optimized multiplex PCR represented the best combination of eleven SSR primers for genotyping common walnut in a single PCR reaction.https://www.silvafennica.fi/article/1674
collection DOAJ
language English
format Article
sources DOAJ
author Ćelepirović, Nevenka
Karija Vlahović, Monika
Dounavi, Aikaterini
Ivanković, Mladen
spellingShingle Ćelepirović, Nevenka
Karija Vlahović, Monika
Dounavi, Aikaterini
Ivanković, Mladen
Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.)
Silva Fennica
author_facet Ćelepirović, Nevenka
Karija Vlahović, Monika
Dounavi, Aikaterini
Ivanković, Mladen
author_sort Ćelepirović, Nevenka
title Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.)
title_short Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.)
title_full Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.)
title_fullStr Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.)
title_full_unstemmed Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>Juglans regia</i> L.)
title_sort optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (<i>juglans regia</i> l.)
publisher Finnish Society of Forest Science
series Silva Fennica
issn 2242-4075
publishDate 2016-01-01
description Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube. In this study, we combined existing simple sequence repeat (SSR) markers (nuclear microsatellites) in the novel combination of multiplex PCR to study the population genetics of common walnut from Croatia. From twenty one tested SSR markers, eleven produced satisfactory results in one multiplex PCR. Population genetic results achieved from 15 samples of Croatian common walnut showed moderate genetic variability (average value: He 0.473; Ho 0.568). Our multiplex PCR allowed cost effective work concerning chemicals, plastic ware, device, and working time producing optimal results. The optimized multiplex PCR represented the best combination of eleven SSR primers for genotyping common walnut in a single PCR reaction.
url https://www.silvafennica.fi/article/1674
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