Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.

Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.

We report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDR...

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Bibliographic Details
Main Authors: John D Morlan, Kunbin Qu, Dominick V Sinicropi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3416766?pdf=render

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