Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.

Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.

We report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDR...

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Main Authors: John D Morlan, Kunbin Qu, Dominick V Sinicropi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3416766?pdf=render
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spelling doaj-c1f0a3e42e634d50aef8e668dbb0df3c2020-11-25T01:46:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4288210.1371/journal.pone.0042882Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.John D MorlanKunbin QuDominick V SinicropiWe report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDRNA removes 98% of targeted RNAs while preserving relative abundance profiles of non-targeted RNAs, enabling routine whole transcriptome analysis of clinically valuable archival tissue specimens by Next-Generation Sequencing.http://europepmc.org/articles/PMC3416766?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author John D Morlan
Kunbin Qu
Dominick V Sinicropi
spellingShingle John D Morlan
Kunbin Qu
Dominick V Sinicropi
Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.
PLoS ONE
author_facet John D Morlan
Kunbin Qu
Dominick V Sinicropi
author_sort John D Morlan
title Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.
title_short Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.
title_full Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.
title_fullStr Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.
title_full_unstemmed Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.
title_sort selective depletion of rrna enables whole transcriptome profiling of archival fixed tissue.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2012-01-01
description We report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDRNA removes 98% of targeted RNAs while preserving relative abundance profiles of non-targeted RNAs, enabling routine whole transcriptome analysis of clinically valuable archival tissue specimens by Next-Generation Sequencing.
url http://europepmc.org/articles/PMC3416766?pdf=render
work_keys_str_mv AT johndmorlan selectivedepletionofrrnaenableswholetranscriptomeprofilingofarchivalfixedtissue
AT kunbinqu selectivedepletionofrrnaenableswholetranscriptomeprofilingofarchivalfixedtissue
AT dominickvsinicropi selectivedepletionofrrnaenableswholetranscriptomeprofilingofarchivalfixedtissue
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